| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeodomain-interacting protein kinase 4, HIPK4 |
| Entrez GeneID | 147746 |
| WB Predicted band size | 69.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HIPK4 antibody is generated from rabbits immunized with a recombinant protein from human HIPK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HIPK4抗体的3-4条参考文献示例(注：部分文献为假设性描述，实际引用时请核实原文)：
1. **文献名称**：HIPK4 is essential for murine spermatogenesis via mediating histone phosphorylation
**作者**：M.G. Geyer, N. Kotaja, et al.
**摘要**：本研究利用HIPK4特异性抗体，通过免疫组化和Western blot分析，发现HIPK4在精子发生过程中调控组蛋白磷酸化，其缺失导致雄性小鼠不育。
2. **文献名称**：HIPK4 overexpression correlates with poor prognosis in colorectal cancer
**作者**：J. Li, H. Wang, et al.
**摘要**：通过HIPK4抗体对结直肠癌组织进行免疫组织化学染色，发现HIPK4高表达与肿瘤侵袭性和患者生存率下降显著相关。
3. **文献名称**：Development and validation of a novel monoclonal antibody against human HIPK4 for functional studies
**作者**：K. Smith, T. Nakamura, et al.
**摘要**：本研究报道了一种新型HIPK4单克隆抗体的开发，验证了其在免疫沉淀和免疫荧光中的特异性，并应用于HIPK4亚细胞定位研究。
4. **文献名称**：HIPK4 modulates neuronal differentiation through kinase-dependent pathways
**作者**：A. Müller, L. Chen, et al.
**摘要**：利用HIPK4抗体进行神经元分化模型分析，揭示HIPK4通过磷酸化下游靶蛋白调控神经突生长和突触形成。
建议通过PubMed或Google Scholar以“HIPK4 antibody”和具体研究领域(如癌症、生殖生物学)为关键词检索最新文献。
**Background of HIPK4 Antibody**
HIPK4 (Homeodomain-Interacting Protein Kinase 4) is a serine/threonine kinase belonging to the HIPK family, which regulates diverse cellular processes, including cell cycle control, transcriptional regulation, and stress response. HIPK4 is less characterized compared to other HIPK members (HIPK1-3) but shares conserved kinase domains and interaction motifs. It is implicated in modulating signaling pathways such as TGF-β and Wnt, impacting cell proliferation, differentiation, and apoptosis. HIPK4 expression is tissue-specific, with notable roles in the testis, brain, and certain cancers, suggesting involvement in reproductive biology, neurodevelopment, and tumorigenesis.
HIPK4 antibodies are essential tools for studying its expression, localization, and function. They enable detection of endogenous HIPK4 via Western blotting, immunohistochemistry, or immunofluorescence, aiding research into its role in diseases like cancer, neurodegenerative disorders, or infertility. Challenges in HIPK4 antibody development include its low expression levels and homology with other HIPKs, necessitating rigorous validation for specificity. Recent studies highlight HIPK4's potential as a therapeutic target, particularly in cancers with dysregulated kinase activity. Reliable antibodies are critical for advancing mechanistic insights and translational applications.
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