| WB | 1/250-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Eukaryotic translation initiation factor 2-alpha kinase 3, 2.7.11.1, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK |
| Entrez GeneID | 9451 |
| WB Predicted band size | 125.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This EIF2AK3 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 530-850 amino acids from human EIF2AK3. |
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以下是关于EIF2AK3抗体的3篇文献示例(内容基于假设性研究概括，实际文献需通过数据库验证)：
1. **文献名称**：*"EIF2AK3 mutations cause Wolcott-Rallison syndrome by impairing pancreatic β-cell function"*
**作者**：Delepine, M., et al.
**摘要**：该研究通过免疫印迹(Western blot)和免疫荧光技术，利用EIF2AK3特异性抗体揭示了该基因突变导致内质网应激反应异常，进而引起胰腺β细胞功能障碍，最终导致Wolcott-Rallison综合征的发生。
2. **文献名称**：*"PERK/EIF2AK3 signaling regulates proteostasis and survival in response to chronic endoplasmic reticulum stress"*
**作者**：Harding, H.P., et al.
**摘要**：作者使用EIF2AK3(PERK)抗体研究其在慢性内质网应激中的作用，发现PERK通过调控eIF2α磷酸化介导细胞适应应激条件，并影响细胞存活与凋亡平衡。
3. **文献名称**：*"Antibody-based detection of EIF2AK3 phosphorylation as a biomarker for endoplasmic reticulum stress in neurodegenerative diseases"*
**作者**：Moreno, J.A., et al.
**摘要**：该文献开发了一种针对磷酸化EIF2AK3的特异性抗体，用于检测阿尔茨海默病等神经退行性疾病模型中的内质网应激激活状态，验证了其在疾病病理中的生物标志物潜力。
4. **文献名称**：*"EIF2AK3 regulates mitochondrial function in hepatic insulin resistance"*
**作者**：Wang, R., et al.
**摘要**：通过免疫沉淀和免疫组化技术，利用EIF2AK3抗体证明其在肝脏胰岛素抵抗中通过调控线粒体功能及氧化应激通路影响糖代谢稳态。
(注：以上为示例，实际文献需通过PubMed、Google Scholar等平台检索确认。)
The EIF2AK3 antibody targets the eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), also known as PERK (PKR-like endoplasmic reticulum kinase). This serine/threonine kinase is a key regulator of the unfolded protein response (UPR), a cellular stress pathway activated by endoplasmic reticulum (ER) dysfunction. Under ER stress, PERK phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), temporarily halting global protein synthesis while allowing selective translation of stress-responsive genes like ATF4. This mechanism helps restore ER homeostasis or triggers apoptosis if stress persists.
EIF2AK3 antibodies are widely used in research to study UPR dynamics in diseases linked to ER stress, such as diabetes, neurodegenerative disorders, and cancer. They enable detection of PERK expression, phosphorylation status, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Mutations in the EIF2AK3 gene cause Wolcott-Rallison syndrome, a rare autosomal recessive disorder characterized by infantile diabetes and multisystemic complications. Antibodies against EIF2AK3 aid in diagnosing this condition and elucidating its molecular pathology.
Commercial EIF2AK3 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Their specificity is validated through knockout controls or siRNA knockdown experiments. Researchers prioritize antibodies recognizing both inactive and phosphorylated (activated) forms of PERK to assess its role in stress signaling pathways. These tools are critical for exploring therapeutic strategies targeting UPR components in proteostasis-related diseases.
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