Mouse Monoclonal RAB20 Antibody

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     All lanes : Anti-RAB20 Antibody at  1:2000-1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing Hela cells stained with P34069(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34069,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The RAB20 antibody is a valuable tool in cellular and molecular biology research, specifically targeting the RAB20 protein—a member of the Rab GTPase family involved in regulating intracellular membrane trafficking. RAB20 is localized to endosomal and lysosomal compartments, where it plays roles in vesicle formation, cargo sorting, and autophagy. Its unique interaction with other Rab proteins and effectors links it to cellular processes like receptor recycling, pathogen defense, and metabolic regulation. Dysregulation of RAB20 has been implicated in cancers, neurodegenerative disorders, and metabolic diseases, driving interest in its functional characterization.

RAB20 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression levels, subcellular localization, and dynamic changes under experimental conditions. Commercial antibodies are typically developed in hosts like rabbits or mice, with validation for specificity via knockout controls or siRNA silencing. Researchers rely on these antibodies to explore RAB20's role in disease mechanisms, such as its overexpression in certain cancers (e.g., hepatocellular carcinoma) or its involvement in autophagy-lysosomal dysfunction in neurodegeneration. However, variability in antibody performance across species or applications necessitates careful validation for reproducible results.