Mouse Monoclonal ACOX1 Antibody

The ACOX1 antibody is a crucial tool for studying acyl-coenzyme A oxidase 1 (ACOX1), a rate-limiting enzyme in the peroxisomal fatty acid β-oxidation pathway. ACOX1 catalyzes the oxidation of very-long-chain fatty acids (VLCFAs) and branched-chain fatty acids, generating hydrogen peroxide as a byproduct. Expressed predominantly in the liver and kidneys, ACOX1 plays a vital role in lipid metabolism and energy homeostasis. Dysregulation of ACOX1 is linked to metabolic disorders, neurodegenerative diseases, and cancer. For instance, ACOX1 mutations are associated with peroxisome biogenesis disorders like Zellweger syndrome, while its overexpression has been observed in certain cancers. Researchers use ACOX1 antibodies in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression levels, tissue distribution, and subcellular localization. These antibodies aid in elucidating ACOX1's role in metabolic pathways, disease mechanisms, and potential therapeutic targets. Commercial ACOX1 antibodies are typically raised in rabbits or mice, targeting specific epitopes of human or rodent ACOX1. Validation for specificity and cross-reactivity is essential, as isoforms (e.g., ACOX2. ACOX3) and species variations may affect experimental outcomes. Proper controls, like knockout cell lines, are recommended to confirm antibody reliability.