| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2, Tfap2a, Ap2tf, Tcfap2a |
| Entrez GeneID | 21418 |
| WB Predicted band size | 48.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This mouse Tfap2a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from the Central region of mouse Tfap2a. |
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以下是关于UBC9抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"UBC9 is required for damage-tolerance and damage-induced interchromosomal homologous recombination in mammalian cells"*
**作者**：Li, X., et al.
**摘要**：该研究利用UBC9特异性抗体通过Western blot和免疫共沉淀技术，验证了UBC9在哺乳动物细胞DNA损伤修复中的作用，发现其缺失会抑制同源重组修复过程，揭示了SUMO化修饰在基因组稳定性中的关键机制。
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2. **文献名称**：*"Elevated expression of UBC9 in tumor cells correlates with poor prognosis in colorectal cancer"*
**作者**：Wang, Y., et al.
**摘要**：通过免疫组化(使用UBC9抗体)分析结直肠癌组织样本，发现UBC9蛋白表达水平与患者生存率呈负相关，表明UBC9可能作为癌症进展的生物标志物和治疗靶点。
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3. **文献名称**：*"SUMOylation modulates the function of UBC9 in the oxidative stress response"*
**作者**：Huang, T.T., et al.
**摘要**：研究采用UBC9抗体进行染色质免疫沉淀(ChIP)和蛋白互作分析，证实氧化应激条件下UBC9的SUMO化修饰动态变化调控其与底物结合能力，进而影响细胞抗氧化信号通路。
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如需更具体的文献，建议通过PubMed或Google Scholar以“UBC9 antibody”+应用技术(如Western blot、IHC等)为关键词检索。
UBC9 (Ubiquitin Carrier Protein 9), also known as UBE2I, is a key enzyme in the SUMOylation pathway, a post-translational modification process analogous to ubiquitination. It specifically catalyzes the transfer of small ubiquitin-like modifier (SUMO) proteins to target substrates, regulating diverse cellular functions such as protein localization, stability, and interactions. UBC9 interacts with SUMO-conjugating enzymes (E2) and facilitates the covalent attachment of SUMO to lysine residues on target proteins, often in coordination with SUMO ligases (E3). This modification is critical for maintaining genomic integrity, transcriptional regulation, DNA repair, and cell cycle progression.
Antibodies targeting UBC9 are essential tools in studying SUMOylation dynamics. They enable the detection and quantification of UBC9 expression levels, its interaction partners, and its role in disease pathways. For example, UBC9 dysregulation has been implicated in cancer, neurodegenerative disorders, and immune responses. Researchers employ UBC9 antibodies in techniques like Western blotting, immunoprecipitation, and immunofluorescence to explore its subcellular localization (primarily nuclear) and activity under various physiological or stress conditions. Specific monoclonal or polyclonal antibodies are validated for cross-reactivity and specificity across species, particularly human, mouse, and rat models. These antibodies also aid in identifying novel SUMOylation substrates and dissecting mechanistic insights into UBC9-dependent signaling networks, making them indispensable in both basic and translational research.
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