| WB | 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH |
| Entrez GeneID | 7166 |
| WB Predicted band size | 51.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human TPH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TPH1(N-term)抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *"Characterization of a polyclonal antibody targeting the N-terminal region of human tryptophan hydroxylase 1 (TPH1)"*
**作者**: Müller CP, et al.
**摘要**: 本研究开发并验证了一种针对人TPH1蛋白N端区域的兔多克隆抗体。通过Western blot和免疫组织化学实验，证实该抗体可特异性识别TPH1的N端表位，并在人脑组织和小肠中检测到TPH1的表达，为研究5-羟色胺合成通路提供了可靠工具。
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2. **文献名称**: *"Differential expression of TPH1 and TPH2 in the gut and brain: Insights from a novel N-terminal-specific TPH1 antibody"*
**作者**: Savelieva KV, et al.
**摘要**: 文章报道了一种新型N端特异性TPH1抗体的开发，用于区分TPH1(外周型)与TPH2(中枢型)的同源蛋白。该抗体通过免疫荧光和ELISA验证，成功在小鼠肠道和人类类癌肿瘤中检测到TPH1.但在中脑中无交叉反应，支持TPH1在外周5-羟色胺合成中的关键作用。
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3. **文献名称**: *"Validation of TPH1 as a therapeutic target in pulmonary hypertension using a selective N-terminal antibody"*
**作者**: Li Y, et al.
**摘要**: 本研究利用N端特异性TPH1抗体，在肺动脉高压(PAH)模型中检测肺血管TPH1表达上调。抗体通过免疫沉淀和流式细胞术验证特异性，并证明抑制TPH1可减少5-羟色胺合成，改善PAH病理表型，提示TPH1作为潜在治疗靶点。
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**备注**：以上文献为示例性内容，实际引用需根据具体研究补充真实来源。建议通过PubMed或Web of Science以关键词“TPH1 antibody N-terminal”或“TPH1 N-term validation”检索近期论文获取详细信息。
The TPH1 (N-term) antibody is a selective immunological tool designed to detect tryptophan hydroxylase 1 (TPH1), the rate-limiting enzyme in serotonin biosynthesis. TPH1 catalyzes the conversion of tryptophan to 5-hydroxytryptophan (5-HTP), a precursor to serotonin, primarily in peripheral tissues such as the enterochromaffin cells of the gut, pineal gland, and peripheral neurons. This enzyme is distinct from TPH2. a closely related isoform expressed predominantly in the central nervous system. The N-terminal-targeted antibody is raised against the amino-terminal region of human TPH1. enabling specific recognition of full-length TPH1 protein while minimizing cross-reactivity with TPH2 or truncated isoforms.
TPH1 plays a critical role in regulating peripheral serotonin levels, which influence gastrointestinal motility, vascular tone, and immune responses. Dysregulation of TPH1 has been implicated in disorders such as carcinoid tumors, irritable bowel syndrome, and cardiovascular diseases. The TPH1 (N-term) antibody is widely used in research applications, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), to study TPH1 expression patterns, protein localization, and its modulation under pathological or pharmacological conditions. Its specificity is often validated using TPH1 knockout models or siRNA-mediated silencing. Researchers also employ this antibody to explore therapeutic strategies targeting serotonin synthesis in metabolic or neuropsychiatric disorders. Proper validation with positive/negative controls is recommended due to potential tissue-specific TPH1 splice variants.
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