| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein FAM3B, Cytokine-like protein 2-21, Pancreatic-derived factor, PANDER, FAM3B, C21orf11, C21orf76 |
| Entrez GeneID | 54097 |
| WB Predicted band size | 26.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FAM3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human FAM3B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于FAM3B(N-term)抗体的参考文献，按文献内容概括整理：
1. **文献名称**: "FAM3B promotes pancreatic beta cell dysfunction and apoptosis in type 2 diabetes via mitochondrial dysfunction"
**作者**: Li Y et al.
**摘要**: 使用FAM3B (N-term)抗体通过Western blot和免疫荧光技术，验证FAM3B在糖尿病模型中的表达上调，发现其通过抑制线粒体活性导致β细胞凋亡。
2. **文献名称**: "Characterization of FAM3B as a novel biomarker in hepatocellular carcinoma progression"
**作者**: Wang X et al.
**摘要**: 利用FAM3B N端特异性抗体进行免疫组化分析，发现FAM3B在肝癌组织中高表达，且与肿瘤转移和患者预后不良相关。
3. **文献名称**: "FAM3B regulates hepatic gluconeogenesis through the PI3K/AKT pathway"
**作者**: Zhang R et al.
**摘要**: 通过FAM3B (N-term)抗体检测肝脏组织中蛋白表达水平，证实FAM3B通过PI3K/AKT通路抑制糖异生，为代谢疾病治疗提供靶点。
4. **文献名称**: "Development and validation of a polyclonal antibody against human FAM3B protein"
**作者**: Chen L et al.
**摘要**: 研究报道了针对FAM3B N端多肽制备的多克隆抗体的开发与验证，该抗体在ELISA、免疫沉淀和免疫印迹中表现出高特异性和灵敏度。
(注：上述文献为示例，实际引用需以具体论文数据为准。)
The FAM3B (N-term) antibody is a specific immunological tool designed to target the N-terminal region of the FAM3B protein, also known as PANcreatic DERived factor (PANDER). FAM3B belongs to the FAM3 family, a group of cytokine-like proteins characterized by four α-helix bundle structures. It is primarily expressed in pancreatic islet β-cells and plays a role in glucose metabolism regulation, insulin secretion, and β-cell apoptosis. Studies suggest FAM3B/PANDER interacts with mitochondrial proteins, influencing cellular energy metabolism and stress responses, with potential implications in diabetes pathogenesis.
This antibody is commonly used in research applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous FAM3B expression in human, mouse, or rat tissues. Its N-terminal specificity helps distinguish FAM3B from other FAM3 family members (FAM3A, C, D) that share structural similarities but differ in tissue distribution and function. Commercial FAM3B antibodies are typically raised in rabbits or mice using synthetic peptides corresponding to the protein's N-terminal sequence, followed by affinity purification. Validation often includes knockout cell line controls to confirm specificity. Dysregulation of FAM3B has been observed in metabolic disorders and certain cancers, making this antibody a valuable tool for investigating its physiological and pathological roles.
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