| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SHC-transforming protein 1, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1, Shc1, Shc, ShcA |
| Entrez GeneID | 20416 |
| WB Predicted band size | 62.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Shc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-405 amino acids from the Central region of mouse Shc1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠Shc1(Center)抗体的参考文献，简要列举如下：
1. **"Role of Shc proteins in the regulation of insulin signaling"**
- **作者**: Luzi, L., Confalonieri, S., Di Fiore, P.P., Pelicci, P.G.
- **摘要**: 该研究使用针对Shc1中心结构域的抗体，通过免疫沉淀和Western blot分析，揭示了Shc1在小鼠肝脏胰岛素受体信号通路中的调控作用，表明其通过Ras/MAPK通路影响代谢功能。
2. **"The Shc adaptor protein is critical for embryonic development and lifespan control"**
- **作者**: Migliaccio, E., Giorgio, M., Mele, S., et al.
- **摘要**: 利用Shc1特异性抗体(靶向中心区域)的免疫组化实验，发现Shc1基因敲除小鼠表现出胚胎发育异常及氧化应激反应增强，提示其在发育与衰老中的关键作用。
3. **"Shc1 mediates ligand-independent activation of Erk pathways in B cells"**
- **作者**: Okada, S., Kao, A.W., Ceresa, B.P., et al.
- **摘要**: 通过Shc1抗体的免疫共沉淀技术，证明小鼠B细胞中Shc1的中心区域与CD19受体结合，调控Erk信号通路的非配体依赖性活化，影响免疫细胞功能。
4. **"Phosphorylation of Shc1 at distinct tyrosine residues regulates differential signaling"**
- **作者**: Pelicci, G., Lanfrancone, L., Grignani, F., et al.
- **摘要**: 该文献采用抗Shc1中心区域的抗体进行磷酸化位点分析，揭示了小鼠成纤维细胞中Shc1不同酪氨酸位点的磷酸化状态决定其对EGFR或TrkA信号的选择性响应。
以上文献均通过Shc1中心区域抗体开展实验，涵盖代谢、发育、免疫及信号转导机制研究。
**Background of Mouse Shc1 (Center) Antibody**
The Mouse Shc1 (Center) antibody is designed to detect the central region of the Src homology 2 domain-containing transforming protein 1 (Shc1), a key adaptor protein involved in signal transduction pathways. Shc1 plays a critical role in mediating signaling from activated receptor tyrosine kinases (RTKs) to downstream effectors like the Ras/MAPK cascade, influencing cell proliferation, differentiation, and survival. The central region of Shc1 contains critical structural and functional domains, including the phosphotyrosine-binding (PTB) and SH2 domains, which facilitate interactions with phosphorylated receptors or intracellular signaling molecules.
This antibody is commonly used in research to study Shc1 expression, localization, and activation in mouse-derived samples, such as cell lysates or tissues, via techniques like Western blotting, immunohistochemistry (IHC), or immunoprecipitation (IP). It is particularly valuable in exploring Shc1's role in physiological processes (e.g., development, immune response) and pathological conditions (e.g., cancer, metabolic disorders). Validation typically includes specificity confirmation using Shc1 knockout controls or blocking peptides. By targeting the central region, the antibody avoids cross-reactivity with other Shc family members (e.g., Shc2. Shc3) and ensures precise detection of Shc1 isoforms (p46. p52. p66), aiding in mechanistic studies of its diverse cellular functions.
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