| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Spermatid-specific linker histone H1-like protein, HILS1 |
| WB Predicted band size | 25.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HILS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the N-terminal region of human HILS1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HILS1(N-term)抗体的3篇参考文献概览(注：HILS1相关研究有限，以下为模拟示例)：
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1. **文献名称**：*HILS1 is a spermatid-specific linker histone-like protein implicated in chromatin remodeling*
**作者**：Yan W, et al.
**摘要**：本研究首次克隆了HILS1基因，发现其在精子发生后期特异性表达。作者制备了针对HILS1蛋白N端的多克隆抗体，通过免疫荧光证实其定位在成熟精子染色质中，并证明HILS1参与染色质浓缩过程。
2. **文献名称**：*Developmental regulation of HILS1 protein degradation in spermatogenesis*
**作者**：Zhou Y, et al.
**摘要**：研究揭示了HILS1在精子形成中的动态降解机制。利用HILS1(N-term)抗体进行Western blot分析，发现其蛋白水平在精子成熟后期显著下降，与泛素化修饰相关，提示其在染色质稳定性调控中的作用。
3. **文献名称**：*Aberrant expression of HILS1 in testicular germ cell tumors*
**作者**：Kato T, et al.
**摘要**：通过免疫组化分析睾丸生殖细胞肿瘤样本，使用HILS1 N端抗体发现其异常高表达与精原细胞瘤亚型相关，提示HILS1可能作为诊断生物标志物。研究还验证了该抗体在石蜡包埋组织中的适用性。
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**说明**：HILS1研究多集中于生殖生物学领域，上述文献为模拟简化版。实际文献检索建议通过PubMed/Google Scholar使用关键词"HILS1 antibody"或"HILS1 N-terminal epitope"筛选，重点关注抗体验证部分(如KO模型验证、肽段竞争实验等)。
The HILS1 (N-term) antibody is specifically designed to target the N-terminal region of the histone-like protein HILS1. which is predominantly expressed in the testes and plays a critical role in spermatogenesis. HILS1. a member of the transition protein family, is involved in chromatin remodeling during the late stages of spermiogenesis, where it replaces histones and facilitates DNA condensation in developing sperm cells. This protein is essential for proper chromatin packaging, ensuring genomic stability and transcriptional silencing in mature sperm.
The HILS1 (N-term) antibody is widely used in reproductive biology research to study the expression, localization, and function of HILS1 in germ cells. It is employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate HILS1 dynamics during spermatogenesis or in pathological conditions such as male infertility. Studies using this antibody have contributed to understanding epigenetic regulation in gametogenesis and the molecular basis of chromatin reorganization defects.
As a mammalian-specific protein, HILS1's expression profile and structural characteristics make it a potential biomarker for assessing sperm quality or diagnosing fertility-related disorders. The N-terminal-targeting antibody helps distinguish HILS1 from other transition proteins, enabling precise analysis of its role in reproductive health and disease.
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