| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DDB1- and CUL4-associated factor 8-like protein 2, WD repeat-containing protein 42C, DCAF8L2, WDR42C |
| WB Predicted band size | 71.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WDR42C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human WDR42C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WDR42C (N-term)抗体的相关文献示例(内容为虚构模拟，仅供参考)：
1. **文献名称**：*Characterization of WDR42C antibody specificity in mitotic regulation*
**作者**：Lee S, et al.
**摘要**：本研究验证了WDR42C N端抗体的特异性，发现其可识别HeLa细胞中内源性WDR42C蛋白，并揭示该蛋白在有丝分裂纺锤体组装中的作用。
2. **文献名称**：*WDR42C interacts with Aurora kinase B: Evidence from co-immunoprecipitation assays*
**作者**：Zhang Y, et al.
**摘要**：利用WDR42C (N-term)抗体进行免疫共沉淀实验，证明WDR42C与Aurora激酶B在细胞周期调控中存在物理相互作用，影响染色体分离。
3. **文献名称**：*Expression profiling of WDR42C in human cancers using a novel N-terminal antibody*
**作者**：Gupta R, et al.
**摘要**：开发了一种高特异性WDR42C N端抗体，通过免疫组化分析发现WDR42C在乳腺癌组织中表达显著上调，提示其潜在肿瘤标志物功能。
4. **文献名称**：*Functional analysis of WDR42C in ciliogenesis via antibody-mediated knockdown*
**作者**：Chen H, et al.
**摘要**：采用WDR42C N端抗体进行功能研究，发现WDR42C缺失导致纤毛形成缺陷，表明其在纤毛发生中具有关键调控作用。
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**注**：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以“WDR42C antibody”为关键词检索最新论文。若研究较少，可扩展至WDR蛋白家族或相关通路(如细胞周期、信号转导)的文献。
The WDR42C (N-term) antibody is a specialized tool designed to detect the N-terminal region of the WD Repeat Domain 42C (WDR42C) protein, a member of the WD-repeat protein family. These proteins are characterized by conserved WD40 motifs, which form β-propeller structures that mediate protein-protein interactions. WDR42C is implicated in various cellular processes, including chromatin remodeling, transcriptional regulation, and signal transduction, though its precise biological functions remain under investigation. The N-terminal region of WDR42C may play a role in substrate recognition or complex assembly, given the modular nature of WD40 proteins.
This antibody is commonly used in research applications such as Western blotting, immunofluorescence, and immunoprecipitation to study WDR42C expression, localization, and interactions. It is often validated for specificity using techniques like siRNA knockdown or knockout cell lines to confirm target band absence. As WD-repeat proteins share structural homology, the antibody’s design typically focuses on unique epitopes within the N-terminal sequence to minimize cross-reactivity.
Commercial sources of the WDR42C (N-term) antibody often provide validation data across species (e.g., human, mouse) and may use recombinant protein fragments or synthetic peptides as immunogens. Researchers rely on this tool to explore WDR42C’s potential roles in diseases, such as cancer or developmental disorders, where dysregulation of chromatin modifiers or signaling pathways is observed.
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