| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Anoctamin-7, Dresden transmembrane protein of the prostate, D-TMPP, IPCA-5, New gene expressed in prostate, Prostate cancer-associated protein 5, Transmembrane protein 16G, ANO7, NGEP, PCANAP5, TMEM16G |
| Entrez GeneID | 50636 |
| WB Predicted band size | 105.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ANO7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human ANO7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ANO7 (N-term)抗体的3篇参考文献的简要概括：
1. **文献名称**：*Characterization of ANO7 as a Novel Biomarker in Prostate Cancer*
**作者**：Smith J, et al.
**摘要**：研究利用ANO7 (N-term)抗体进行免疫组织化学分析，发现ANO7在前列腺癌组织中高表达，且其N端结构域与肿瘤侵袭性相关，提示其作为潜在诊断标志物的价值。
2. **文献名称**：*Functional Analysis of ANO7 Ion Channel Domains Using Domain-Specific Antibodies*
**作者**：Lee S, et al.
**摘要**：通过Western blot和免疫荧光验证ANO7 (N-term)抗体的特异性，发现N端结构域对ANO7的钙离子通道功能及细胞膜定位具有关键调控作用。
3. **文献名称**：*ANO7 Expression and Localization in Hormone-Refractory Cancers*
**作者**：Garcia R, et al.
**摘要**：利用ANO7 (N-term)抗体检测多种癌症细胞系，发现其在激素治疗抵抗的前列腺癌细胞中异常表达，可能与耐药性机制相关。
以上内容为模拟示例，实际文献需通过学术数据库检索确认。
The ANO7 (N-term) antibody is a reagent designed to target the N-terminal region of the Anoctamin 7 protein (ANO7), also known as TMEM16G, which belongs to the anoctamin family of calcium-activated chloride channels and phospholipid scramblases. ANO7 is a transmembrane protein implicated in ion transport, membrane dynamics, and cellular processes such as apoptosis and secretion. It is notably expressed in prostate tissue and has gained attention as a potential biomarker for prostate cancer due to its upregulated expression in malignant cells compared to normal tissue.
The ANO7 (N-term) antibody is typically developed for research applications, including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), to detect the presence and localization of the ANO7 protein in biological samples. Its specificity for the N-terminal domain ensures recognition of full-length ANO7 isoforms, distinguishing them from truncated variants or degradation products. Validation often involves confirming reactivity in cell lines or tissues with known ANO7 expression and using knockout controls to verify absence of cross-reactivity.
Studies utilizing this antibody have contributed to understanding ANO7's role in prostate cancer progression, where it may influence cell proliferation, migration, and androgen signaling. Additionally, ANO7 has been explored in other contexts, including immune regulation and lipid metabolism. The antibody's utility in both basic research and clinical investigations underscores its importance in elucidating ANO7's functional mechanisms and potential therapeutic applications.
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