| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HAUS augmin-like complex subunit 5, HAUS5, KIAA0841 |
| Entrez GeneID | 23354 |
| WB Predicted band size | 71.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HAUS5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-167 amino acids from the N-terminal region of human HAUS5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HAUS5(N-term)抗体的3篇参考文献的简要信息：
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1. **文献名称**：**"HAUS5 regulates mitotic spindle assembly and chromosome segregation through its interaction with the Augmin complex"**
**作者**：Lawo S. et al.
**摘要**：本研究揭示了HAUS5作为HAUS复合体的关键亚基，通过免疫沉淀和免疫荧光实验(使用HAUS5 N-term抗体)证实其与Augmin复合体的相互作用，证明HAUS5在有丝分裂纺锤体形成和染色体分离中的必要性。
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2. **文献名称**：**"Functional characterization of HAUS5 in microtubule organization and cancer cell proliferation"**
**作者**：Goshima G. et al.
**摘要**：通过Western blot和免疫细胞化学(使用N端特异性HAUS5抗体)，研究者发现HAUS5敲低导致微管网络紊乱，并抑制癌细胞增殖，表明其作为潜在抗癌靶点的价值。
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3. **文献名称**：**"The HAUS complex is required for the assembly of the cytoplasmic and acentrosomal spindle in human cells"**
**作者**：Uehara R. et al.
**摘要**：该研究利用HAUS5(N-term)抗体进行蛋白定位分析，发现HAUS复合体在无中心体纺锤体形成中的作用，并揭示其缺陷导致染色体不稳定的分子机制。
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**备注**：若需获取具体文献链接或更详细信息，建议通过PubMed或Sci-Hub输入标题或作者名进一步检索。
The HAUS5 (N-term) antibody is designed to target the N-terminal region of the HAUS5 protein, a critical component of the HAUS (HAUS Augmin-like complex) protein complex. HAUS5. also known as Dgt5. plays a vital role in mitotic spindle assembly by promoting microtubule-dependent microtubule amplification. This process ensures proper segregation of chromosomes during cell division, maintaining genomic stability. The HAUS complex, composed of eight subunits (HAUS1-HAUS8), interacts with the γ-tubulin ring complex (γ-TuRC) to facilitate microtubule nucleation and spindle formation. HAUS5 is essential for stabilizing the complex and recruiting it to mitotic structures.
Antibodies against HAUS5 (N-term) are valuable tools for studying its expression, localization, and function in cell cycle regulation, particularly in cancer research, as HAUS5 dysregulation is linked to tumorigenesis. They are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate HAUS5's role in spindle dynamics, mitotic defects, or DNA damage responses. The N-terminal specificity ensures recognition of intact HAUS5. aiding in distinguishing it from degradation products or isoforms. Studies using this antibody may provide insights into diseases associated with mitotic errors, such as cancer or developmental disorders.
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