Rabbit Polyclonal RPS7 Antibody

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     All lanes : Anti-RPS7 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling RPS7 with P33950 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG  secondary antibody at 1/200 dilution (green). Immunofluorescence image mainly showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing Hela cells stained with P33950 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33950,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The RPS7 antibody targets ribosomal protein S7. a component of the 40S ribosomal subunit involved in mRNA translation. RPS7. encoded by the *RPS7* gene, not only plays a critical role in ribosome biogenesis and protein synthesis but also participates in extra-ribosomal functions, including DNA repair, apoptosis regulation, and cellular stress responses. Dysregulation of RPS7 has been linked to cancers, neurodegenerative disorders, and Diamond-Blackfan anemia (DBA), a rare ribosomopathy caused by mutations in ribosomal protein genes.

RPS7 antibodies are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. In research, they are used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to analyze RPS7 levels in tissues or cell lines, particularly in cancer models where its overexpression may correlate with tumor progression. Additionally, these antibodies help investigate RPS7's role in ribosomal stress pathways and its interaction with molecules like MDM2/p53. which regulates cell cycle arrest under ribosomal dysfunction. Commercial RPS7 antibodies are typically developed in rabbit or mouse hosts, validated for specificity via knockout controls. Challenges include cross-reactivity with homologous proteins, necessitating careful validation. Overall, RPS7 antibodies provide insights into ribosome-related diseases and potential therapeutic targets.