| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2 |
| Entrez GeneID | 10403 |
| WB Predicted band size | 73.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-539 amino acids from human NDC80. |
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以下是关于NDC80抗体的3篇参考文献，涵盖其功能研究及实验应用：
1. **文献名称**：*"Architecture and Flexibility of the Ndc80 Complex’s Ectodomain in Human Kinetochores"*
**作者**：Ciferri, C. et al.
**摘要**：通过冷冻电镜和抗体标记技术解析人源NDC80复合体的三维结构，揭示其与微管结合的分子机制，抗体被用于定位复合体在动粒上的空间分布。
2. **文献名称**：*"The NDC80 Complex Bridges the Core Kinetochore to Dynamic Microtubule Tips"*
**作者**：Cheeseman, I.M. et al.
**摘要**：利用NDC80特异性抗体进行RNA干扰和免疫荧光实验，证明该复合体是连接动粒核心与微管末端动态附着的关键桥梁，确保染色体精准分离。
3. **文献名称**：*"Phosphorylation Controls Spatial and Temporal Activities of the NDC80 Complex in Mitosis"*
**作者**：Guimaraes, G.J. et al.
**摘要**：通过磷酸化特异性抗体分析NDC80的翻译后修饰，阐明其磷酸化状态如何调控动粒-微管附着的稳定性及细胞周期检查点应答。
4. **文献名称**：*"Aurora B Phosphorylates Spatially Distinct Targets to Coordinate Kinetochore-Microtubule Interactions"*
**作者**：DeLuca, K.F. et al.
**摘要**：结合NDC80抗体的免疫沉淀和活细胞成像技术，揭示Aurora B激酶通过磷酸化NDC80调控动粒张力感知与错误附着的纠正机制。
*注：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对详细信息及可用性。*
The NDC80 antibody targets the NDC80 protein, a critical component of the evolutionarily conserved NDC80 complex, which plays an essential role in chromosome segregation during mitosis. The NDC80 complex (comprising NDC80/HEC1. Nuf2. Spc24. and Spc25) forms a key part of the kinetochore-microtubule interface, mediating stable attachments between spindle microtubules and kinetochores to ensure accurate chromosome alignment and segregation. Dysregulation of this complex can lead to mitotic errors, genomic instability, and aneuploidy, hallmarks of cancer and developmental disorders.
NDC80 antibodies are widely used in cell biology research to study mitotic mechanisms, kinetochore architecture, and microtubule-kinetochore dynamics. They enable visualization of NDC80 localization via immunofluorescence, quantification of protein expression by Western blot, or assessment of kinetochore function in live-cell imaging. Additionally, these antibodies are valuable tools in cancer research, as NDC80 overexpression correlates with tumor progression and poor prognosis in certain cancers, suggesting its potential as a therapeutic target. Antibodies are available in polyclonal or monoclonal formats, often validated for specificity in model organisms (e.g., human, mouse) and compatibility with techniques like ChIP-seq or super-resolution microscopy. Researchers also utilize NDC80 inhibitors or RNAi alongside antibodies to explore functional roles in mitotic regulation and disease pathways.
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