| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1, NUP85, NUP75, PCNT1 |
| Entrez GeneID | 79902 |
| WB Predicted band size | 75.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NUP85 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-195 amino acids from human NUP85. |
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以下是关于NUP85 (N-Term)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural and functional analysis of the NUP107-160 complex in nuclear pore assembly*
**作者**：Walther TC, et al.
**摘要**：该研究通过免疫沉淀和质谱分析揭示了NUP85(N端抗体用于检测)在核孔复合体亚基NUP107-160中的关键作用，证明其参与核膜重建和有丝分裂调控。
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2. **文献名称**：*NUP85 mutations disrupt the nuclear pore complex and cause steroid-resistant nephrotic syndrome*
**作者**：Braun DA, et al.
**摘要**：文章利用NUP85 N端特异性抗体进行免疫荧光染色，发现患者细胞中NUP85表达异常，导致核孔复合体结构缺陷，与肾病变相关。
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3. **文献名称**：*Antibody-based profiling of nucleoporin dynamics during interphase and mitosis*
**作者**：Dultz E, Ellenberg J.
**摘要**：研究通过NUP85 N端抗体实时观察细胞周期中核孔蛋白的动态变化，发现其在有丝分裂解体后于子细胞核膜上重新组装的过程。
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以上文献均使用NUP85 N端抗体进行蛋白定位、功能研究或疾病关联分析，涵盖结构、动态及临床相关机制。若需具体文献链接或更多细节，可进一步补充检索条件。
The NUP85 (N-Term) antibody targets the N-terminal region of Nucleoporin 85 (NUP85), a critical component of the nuclear pore complex (NPC). NUP85 is part of the Nup107-160 subcomplex, which forms the structural scaffold of the NPC and facilitates nucleocytoplasmic transport of macromolecules. This multi-protein complex is essential for maintaining nuclear envelope integrity, regulating gene expression, and mediating cellular processes such as mitosis and DNA repair. The N-terminal domain of NUP85 plays a role in interactions with other nucleoporins, including NUP107 and NUP133. ensuring proper assembly and stability of the NPC.
Antibodies against NUP85 (N-Term) are widely used in research to study NPC architecture, dynamics, and dysfunction in diseases. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, localization, and interactions. Aberrant expression or mutations in NUP85 have been linked to pathologies such as cancer, cardiovascular disorders, and rare genetic conditions like steroid-resistant nephrotic syndrome. By enabling precise detection of NUP85. these antibodies contribute to understanding the role of nucleoporins in cellular homeostasis and disease mechanisms. Commercial NUP85 (N-Term) antibodies are typically validated for specificity and cross-reactivity across human, mouse, and rat models.
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