| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ret finger protein-like 3, RFPL3 |
| Entrez GeneID | 10738 |
| WB Predicted band size | 35.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RFPL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human RFPL3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RFPL3 (N-term)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *RFPL3 interacts with CASC5 and regulates cancer cell proliferation*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用RFPL3 (N-term)抗体进行免疫沉淀和Western blot分析，发现RFPL3与染色体组装蛋白CASC5直接相互作用，并通过调控细胞周期蛋白表达影响肝癌细胞增殖。研究强调了RFPL3在肿瘤发生中的潜在作用。
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2. **文献名称**: *Characterization of RFPL3 in neural progenitor cell differentiation*
**作者**: Lee S, et al.
**摘要**: 通过RFPL3 (N-term)抗体的免疫荧光实验，作者证明RFPL3在小鼠胚胎神经祖细胞中高表达，并参与Notch信号通路的调控。敲低RFPL3导致神经元分化异常，提示其在神经发育中的功能。
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3. **文献名称**: *A novel role of RFPL3 in DNA damage response*
**作者**: Gupta R, et al.
**摘要**: 该研究使用RFPL3特异性抗体(针对N端表位)进行染色质免疫共沉淀(ChIP)，发现RFPL3在DNA损伤后募集至断裂位点，与ATM激酶协同促进修复。结果为RFPL3在基因组稳定性中的作用提供了新证据。
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4. **文献名称**: *Antibody validation for RFPL3 in human tissue microarrays*
**作者**: Müller H, et al.
**摘要**: 本文系统验证了RFPL3 (N-term)抗体的特异性，通过敲除细胞系和多种组织样本的免疫组化分析，证实该抗体在检测人卵巢癌和正常组织中RFPL3蛋白表达的可靠性，并建立其临床病理关联。
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以上文献均涉及RFPL3抗体的实验应用，涵盖癌症、发育生物学及抗体验证等领域。如需具体文献链接或补充信息，可进一步说明。
The RFPL3 (N-term) antibody is a targeted immunological tool designed to detect the N-terminal region of the Retfinger Protein-Like 3 (RFPL3), a member of the RFPL gene family. This family, primarily expressed in germline cells, is implicated in gametogenesis and meiotic processes. RFPL3. specifically, is thought to regulate chromatin dynamics and transcriptional activity during germ cell development, though its exact mechanisms remain under investigation. The antibody’s design focuses on the N-terminal domain due to its potential role in mediating protein-protein interactions or post-translational modifications critical for RFPL3’s function.
Researchers utilize this antibody in applications like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF) to study RFPL3 expression patterns, particularly in reproductive tissues or germ cell-derived models. Its specificity for the N-terminal region ensures recognition of full-length RFPL3 while avoiding cross-reactivity with truncated isoforms or homologous proteins (e.g., RFPL1. RFPL2). Validation often includes testing in knockout (KO) models or siRNA-treated samples to confirm target specificity. Dysregulation of RFPL3 has been loosely associated with infertility and developmental disorders, making this antibody a valuable tool for exploring reproductive biology and disease mechanisms. Optimal performance typically requires antigen retrieval in IHC due to the protein’s nuclear localization and tight chromatin binding.
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