Rabbit Polyclonal SCRIB(N-term) Antibody

The SCRIB (N-term) antibody targets the N-terminal region of the SCRIB (Scribble) protein, a critical scaffold protein involved in regulating cell polarity, migration, and proliferation. SCRIB belongs to the LAP (LRR and PDZ domain-containing) protein family and plays a key role in maintaining tissue architecture by localizing to cell membrane domains, such as tight junctions and adherens junctions. It interacts with multiple signaling pathways, including Hippo, Wnt/β-catenin, and MAPK/ERK, to control cell fate decisions and tissue homeostasis. Dysregulation of SCRIB is linked to cancer, where it can act as a tumor suppressor or oncogene depending on cellular context. The N-terminal region of SCRIB contains leucine-rich repeats (LRRs) crucial for protein-protein interactions, while its C-terminal PDZ domains mediate binding to downstream effectors. The SCRIB (N-term) antibody is commonly used in research to detect full-length SCRIB or specific isoforms in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Its specificity for the N-terminus helps distinguish SCRIB from related family members (e.g., DLG1. DLG5) and ensures accurate localization studies. Researchers often validate this antibody in SCRIB-knockout models to confirm target specificity, as cross-reactivity with other LAP proteins remains a potential concern.