| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DnaJ homolog subfamily A member 2, Cell cycle progression restoration gene 3 protein, Dnj3, Dj3, HIRA-interacting protein 4, Renal carcinoma antigen NY-REN-14, DNAJA2, CPR3, HIRIP4 |
| Entrez GeneID | 10294 |
| WB Predicted band size | 45.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DNAJA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-72 amino acids from the N-terminal region of human DNAJA2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及DNAJA2(N-term)抗体的文献摘要信息：
1. **文献名称**：*DNAJA2 modulates the secretory pathway in cancer cells*
**作者**：Smith A et al.
**摘要**：该研究利用抗DNAJA2(N端)抗体进行免疫印迹和免疫荧光实验，发现DNAJA2通过与内质网相关蛋白互作调控肿瘤细胞中异常蛋白的分泌通路，影响癌症转移。
2. **文献名称**：*Hsp40 family member DNAJA2 regulates mitochondrial protein quality control*
**作者**：Chen L et al.
**摘要**：通过DNAJA2(N-term)抗体的免疫共沉淀技术，作者揭示了DNAJA2在线粒体应激条件下参与错误折叠蛋白的识别与清除，并影响细胞凋亡信号通路。
3. **文献名称**：*DNAJA2 as a novel biomarker for neurodegenerative diseases*
**作者**：Wang Y et al.
**摘要**：该研究使用DNAJA2(N端特异性)抗体检测阿尔茨海默病模型小鼠脑组织，发现DNAJA2在神经元突触中的异常聚集与tau蛋白病理相关，提示其作为潜在治疗靶点。
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注：以上文献为示例，实际文献需根据具体数据库检索。若需真实文献，建议通过PubMed或Web of Science以“DNAJA2 AND (antibody OR N-terminal)”为关键词筛选。
The DNAJA2 (N-term) antibody is a specialized tool used to detect the N-terminal region of the DNAJA2 protein, a member of the Hsp40/DnaJ family. DNAJA2 functions as a co-chaperone, collaborating with Hsp70 to regulate protein folding, disaggregation, and degradation. It plays a critical role in cellular stress responses, ensuring proteostasis by stabilizing nascent polypeptides, preventing aggregation, and facilitating the refolding of misfolded proteins. The N-terminal domain of DNAJA2 contains a conserved J-domain responsible for binding Hsp70. while its C-terminal region interacts with client proteins.
This antibody is commonly utilized in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study DNAJA2 expression, localization, and interactions under physiological or stress conditions. Researchers employ it to explore DNAJA2's involvement in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, where chaperone dysfunction is implicated. Specificity validation, including knockdown/knockout controls, is essential due to high homology among DnaJ family members (e.g., DNAJA1. DNAJA3). Studies suggest DNAJA2 may serve as a biomarker or therapeutic target, particularly in cancers with dysregulated protein homeostasis. Its mitochondrial localization in certain contexts also links it to energy metabolism and apoptosis pathways. Proper experimental optimization (e.g., sample preparation, antibody dilution) is crucial for reliable results.
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