Mouse Monoclonal TERF2IP Antibody

+ +

•  

   
     All lanes : Anti-TERF2IP Antibody at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     Overlay histogram showing Hela cells stained with P33896(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33896,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

•  

   
     P33896 staining TE2IP in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The TERF2IP antibody targets the TERF2 Interacting Protein (TERF2IP), also known as Rap1. a critical component of the shelterin complex responsible for telomere maintenance and genome stability. TERF2IP binds directly to TRF2. another shelterin protein, and plays a dual role in telomere protection and transcriptional regulation. It facilitates telomere looping by tethering telomeres to the nuclear matrix and participates in the repression of homology-directed repair (HDR) to prevent telomere fusions. Beyond telomere biology, TERF2IP interacts with NF-κB signaling pathways, influencing inflammatory responses and apoptosis.

Antibodies against TERF2IP are widely used in research to study telomere dynamics, DNA damage response, and chromatin organization. They enable detection of TERF2IP expression via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Dysregulation of TERF2IP is linked to aging-related diseases, cancer (due to telomere dysfunction), and rare genetic disorders like dyskeratosis congenita. These antibodies also aid in exploring TERF2IP's non-telomeric roles, such as its involvement in transcriptional regulation and immune signaling. Commercial TERF2IP antibodies are typically validated for specificity using knockout cell lines or siRNA knockdowns to ensure reliable experimental outcomes.