| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tyrosine-protein kinase RYK, 2.7.10.1, RYK, JTK5A |
| Entrez GeneID | 6259 |
| WB Predicted band size | 67.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RYK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 260-565 amino acids from human RYK. |
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以下是关于RYK抗体的3篇参考文献，涵盖其在不同研究中的应用和功能分析：
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1. **文献名称**：*RYK as a receptor for Wnt ligands in neural development*
**作者**：Yoshikawa S, et al.
**摘要**：该研究通过Western blot和免疫组化实验，使用RYK特异性抗体揭示了RYK在哺乳动物大脑发育中的关键作用。研究发现，RYK作为非经典Wnt信号受体，调控神经前体细胞的迁移与分化，抗体验证了其在神经元轴突导向中的表达定位。
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2. **文献名称**：*RYK-mediated Wnt signaling in colorectal cancer progression*
**作者**：Zhang L, et al.
**摘要**：本文利用RYK抗体(克隆号：EPR1292)分析结直肠癌组织样本，发现RYK蛋白在肿瘤侵袭前沿高表达，且与患者预后不良相关。通过敲低实验和抗体介导的功能阻断，证实RYK通过激活β-catenin非依赖性通路促进癌细胞转移。
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3. **文献名称**：*Antibody-based targeting of RYK inhibits tumor angiogenesis*
**作者**：Kim H, et al.
**摘要**：研究开发了一种人源化抗RYK单克隆抗体(anti-RYK mAb)，并在小鼠模型中验证其抑制肿瘤血管生成的效果。实验显示，该抗体通过阻断RYK与Wnt5a的相互作用，显著减少内皮细胞增殖和血管形成，为癌症治疗提供新靶点策略。
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4. **文献名称**：*RYK regulates planar cell polarity in Drosophila via antibody-assisted localization studies*
**作者**：Gonzalez-Perez E, et al.
**摘要**：通过果蝇模型，结合抗RYK抗体的免疫荧光技术，研究发现RYK在体节极性形成中调控平面细胞极性(PCP)。抗体特异性检测到RYK在上皮细胞顶膜区的极性分布，证实其与Frizzled受体协同作用维持组织形态。
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以上文献展示了RYK抗体在神经发育、癌症机制及发育生物学中的关键应用，涉及蛋白定位、功能验证及治疗开发等领域。
RYK (Receptor-like Tyrosine Kinase) is a unique member of the receptor tyrosine kinase (RTK) family, distinguished by its catalytically inactive pseudokinase domain. Initially identified in the 1990s, RYK functions as a co-receptor in Wnt signaling pathways, particularly non-canonical Wnt pathways, playing critical roles in embryonic development, tissue patterning, and neural connectivity. Unlike typical RTKs, RYK lacks intrinsic kinase activity due to amino acid substitutions in its kinase domain, relying instead on interactions with other signaling molecules, such as Dishevelled, to mediate downstream effects.
Structurally, RYK contains an extracellular leucine-rich repeat (LRR) domain, a transmembrane region, and an intracellular pseudokinase domain. It is implicated in axon guidance, cell migration, and synaptogenesis, with studies linking RYK dysregulation to neurodevelopmental disorders, cancer progression, and neurodegenerative diseases. For instance, aberrant RYK expression has been observed in glioblastoma and breast cancer, suggesting its role in tumor invasiveness.
RYK antibodies are essential tools for detecting RYK expression, localization, and post-translational modifications in research. They enable investigations into RYK's interaction networks and its context-dependent signaling mechanisms, offering insights into therapeutic targeting for diseases associated with Wnt pathway dysregulation. Despite its "inactive" kinase classification, RYK remains a pivotal player in developmental and pathological processes, driving ongoing interest in its biology.
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