Mouse Monoclonal RAB1B Antibody

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     All lanes : Anti-RAB1B Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-20S whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing A431 cells stained with P33891(green line).   The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.   The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33891,   1:25 dilution) for 60 min at 37ºC.   The secondary antibody used was Goat-Anti-Mouse IgG,  DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC.   Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions.   Acquisition of >10,  000 events was performed.    

The RAB1B antibody is a crucial tool for studying the RAB1B protein, a member of the Ras-related GTPase superfamily involved in regulating intracellular vesicular trafficking. RAB1B plays a pivotal role in endoplasmic reticulum (ER)-to-Golgi transport, facilitating the docking and fusion of COPII-coated vesicles with the Golgi apparatus. It is implicated in maintaining Golgi structure, autophagy, and secretory pathways, with dysregulation linked to cancers, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s diseases), and infectious pathogen propagation.

RAB1B antibodies are typically generated using immunogens such as recombinant proteins or synthetic peptides corresponding to specific epitopes of human RAB1B. These antibodies are widely used in techniques like Western blotting, immunofluorescence (IF), and immunohistochemistry (IHC) to detect RAB1B expression, localization, and interactions in cell lines or tissues. Specificity is validated via knockout controls or siRNA-mediated silencing.

Commercially available RAB1B antibodies are offered by major suppliers (e.g., Cell Signaling Technology, Abcam, Sigma-Aldrich) and often cite peer-reviewed studies. Researchers rely on these reagents to explore RAB1B's functional roles in membrane trafficking dynamics, disease mechanisms, and therapeutic targeting. Cross-reactivity with homologs (e.g., RAB1A) should be assessed, as sequence similarity within the RAB family may vary. Proper validation ensures accurate interpretation of experimental results in both basic and translational research contexts.