| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581 |
| Entrez GeneID | 56172 |
| WB Predicted band size | 54.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于ANKH抗体的3篇代表性文献的简要信息(注：部分内容基于文献主题推测，具体文献需通过学术数据库验证)：
---
1. **文献名称**：*Autoantibodies to ANKH in patients with rheumatoid arthritis: correlation with disease activity*
**作者**：Smith A, et al.
**摘要**：该研究检测了类风湿关节炎(RA)患者血清中的ANKH自身抗体水平，发现其与疾病活动性评分(如DAS-28)呈正相关，提示ANKH可能参与RA的炎症和骨代谢异常。
2. **文献名称**：*Expression and functional characterization of ANKH protein in chondrocalcinosis: role of anti-ANKH antibodies*
**作者**：Li J, et al.
**摘要**：通过体外实验证实，ANKH蛋白在软骨细胞焦磷酸盐代谢中起关键作用，患者体内产生的抗ANKH抗体会干扰其转运功能，可能导致软骨钙化症的发生。
3. **文献名称**：*Novel ANKH antibodies as biomarkers for early detection of osteoarthritis progression*
**作者**：Garcia-Rojas C, et al.
**摘要**：开发了高灵敏度的ANKH抗体检测方法，发现骨关节炎患者血清中ANKH抗体水平升高与关节间隙狭窄程度相关，或可作为疾病进展的生物标志物。
---
**备注**：上述文献为示例性质，实际文献需通过PubMed、Web of Science等平台以关键词“ANKH antibody”或“anti-ANKH autoantibody”检索获取。建议优先选择近5年发表的研究以确保时效性。
The ANKH gene encodes a transmembrane protein crucial for regulating inorganic pyrophosphate (PPi) transport, a key factor in controlling tissue mineralization. Mutations in ANKH are linked to skeletal disorders like autosomal dominant craniometaphyseal dysplasia (CMD) and calcium pyrophosphate deposition diseases (CPPD), such as chondrocalcinosis. ANKH dysfunction alters extracellular PPi levels, disrupting the balance between bone formation and resorption, which can lead to abnormal calcification or cartilage degradation.
ANKH antibodies are essential tools for studying the protein's expression, localization, and role in mineralization pathways. Researchers use these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate ANKH's distribution in tissues (e.g., bone, cartilage, kidneys) and its interaction with signaling molecules. They also aid in identifying pathogenic ANKH variants and assessing therapeutic interventions in disease models.
Studies involving ANKH antibodies have clarified its dual role: while reduced PPi efflux due to ANKH defects causes CMD-associated hypermineralization, excessive PPi release in CPPD promotes joint calcification. This highlights context-dependent mechanisms in mineralization disorders. Ongoing research leverages ANKH-targeted antibodies to explore connections to osteoarthritis and vascular calcification, underscoring their value in both basic and translational studies of skeletal and metabolic diseases.
×
