| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Glycine dehydrogenase (decarboxylating), mitochondrial, Glycine cleavage system P protein, Glycine decarboxylase, Glycine dehydrogenase (aminomethyl-transferring), GLDC, GCSP |
| Entrez GeneID | 2731 |
| WB Predicted band size | 112.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This GLDC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-77 amino acids from the N-terminal region of human GLDC. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于GLDC(N-term)抗体的可能研究方向和文献示例的模拟参考(注：以下为示例性内容，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Glycine Decarboxylase (GLDC) Expression in Non-Ketotic Hyperglycinemia: Role of N-Terminal Antibodies in Pathogenic Variant Detection"*
**作者**：Smith A, et al.
**摘要**：本研究利用针对GLDC蛋白N端的特异性抗体，通过Western blot和免疫荧光技术，分析了非酮症性高甘氨酸血症(NKH)患者细胞中GLDC的异常表达。结果揭示了N端抗体在检测致病性截短突变中的高灵敏性，为临床诊断提供了工具支持。
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2. **文献名称**：*"GLDC-Driven Glycine Metabolism Promotes Lung Cancer Progression: Insights from Antibody-Based Detection"*
**作者**：Chen L, et al.
**摘要**：该研究通过抗GLDC N端抗体验证了GLDC在肺癌组织中的过表达，并发现其与甘氨酸代谢异常和肿瘤细胞增殖相关。抗体特异性通过siRNA敲低实验确认，为靶向甘氨酸代谢的癌症治疗提供了依据。
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3. **文献名称**：*"Characterization of a Novel GLDC (N-Term) Monoclonal Antibody for Mitochondrial Protein Localization Studies"*
**作者**：Yamamoto K, et al.
**摘要**：文章报道了一种新型GLDC N端单克隆抗体的开发，通过免疫组化和线粒体分离实验证实其对内源性GLDC蛋白的特异性识别，适用于研究GLDC在线粒体代谢中的亚细胞定位及功能。
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4. **文献名称**：*"GLDC Gene Variants and Protein Stability in Neural Development: Insights from Antibody-Based Assays"*
**作者**：Gupta R, et al.
**摘要**：利用N端抗体检测GLDC蛋白稳定性，发现特定错义突变导致其降解加速，可能与神经发育异常相关。研究强调了该抗体在功能基因组学中的应用潜力。
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**注意事项**：
- 以上文献为模拟示例，具体研究需参考真实发表的论文。
- 建议通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词如 **"GLDC antibody N-terminal"**、**"glycine decarboxylase immunoassay"** 获取最新文献。
- 部分厂商(如Abcam、CST)的抗体说明书可能提供相关应用文献，可访问其官网查询。
如需进一步协助文献检索，请提供更多信息(如研究领域或实验目的)。
The GLDC (N-term) antibody targets the N-terminal region of the glycine decarboxylase (GLDC) protein, a key component of the mitochondrial glycine cleavage system (GCS). GLDC catalyzes the decarboxylation of glycine, a critical step in glycine metabolism, linking amino acid catabolism to one-carbon metabolism and energy production. This enzyme is highly expressed in the liver, kidney, and brain, where it helps regulate glycine levels and contributes to folate-mediated processes.
Mutations in the GLDC gene are associated with nonketotic hyperglycinemia (NKH), a rare autosomal recessive disorder characterized by glycine accumulation, leading to severe neurological impairments. Studying GLDC expression and function is thus vital for understanding NKH pathogenesis and developing therapeutic strategies. The GLDC (N-term) antibody is widely used in research to detect GLDC protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Its specificity for the N-terminal region ensures recognition of full-length GLDC, helping distinguish it from potential degradation products or isoforms.
Researchers also employ this antibody to investigate GLDC's role in cellular metabolism, cancer (where glycine metabolism may influence tumor progression), and neurological disorders. By enabling precise localization and quantification of GLDC, the antibody supports studies on tissue-specific expression patterns and molecular mechanisms underlying GLDC-related diseases. Its utility extends to validating gene-editing models (e.g., CRISPR/Cas9) and assessing therapeutic interventions targeting glycine metabolic pathways.
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