| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Insulin receptor substrate 2, IRS-2, IRS2 |
| Entrez GeneID | 8660 |
| WB Predicted band size | 137.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇关于IRS2抗体的参考文献，简要概括如下：
1. **文献名称**: *"IRS-2 coordinates Igf-1 receptor-mediated β-cell development and peripheral insulin signalling"*
**作者**: Kubota, N. et al.
**摘要**: 该研究利用IRS2抗体检测小鼠β细胞中IRS2蛋白表达，揭示IRS2在胰岛素样生长因子-1(IGF-1)信号通路中的作用，调控β细胞增殖及外周胰岛素敏感性。
2. **文献名称**: *"Role of IRS-2 in insulin and cytokine signalling"*
**作者**: Biddinger, S.B. & Kahn, C.R.
**摘要**: 通过IRS2抗体进行Western blot分析，发现IRS2缺失导致肝脏胰岛素抵抗，强调其在介导胰岛素和细胞因子信号转导中的关键性，与代谢综合征相关。
3. **文献名称**: *"Differential expression of IRS-1 and IRS-2 in breast cancer cells exposed to hyperinsulinemia"*
**作者**: Taguchi, A. et al.
**摘要**: 使用IRS2抗体比较乳腺癌细胞系中IRS1和IRS2的表达差异，发现高胰岛素血症通过IRS2而非IRS1促进癌细胞增殖，提示其潜在致癌机制。
4. **文献名称**: *"Impaired insulin signaling in Alzheimer’s disease: IRS-2 phosphorylation and subcellular localization"*
**作者**: De la Monte, S.M. & Wands, J.R.
**摘要**: 通过免疫组化结合IRS2抗体，发现阿尔茨海默病患者脑组织中IRS2磷酸化异常，提示胰岛素信号失调与神经退行性病变的关联。
以上文献均通过IRS2抗体探究其在代谢、癌症及神经疾病中的分子机制。如需具体期刊信息或年份，可进一步补充查询。
The insulin receptor substrate 2 (IRS2) antibody is a crucial tool in biomedical research for studying the role of IRS2. a key adaptor protein in insulin and insulin-like growth factor (IGF) signaling pathways. IRS2 mediates intracellular signaling by binding to activated insulin/IGF receptors, facilitating downstream phosphorylation events that regulate glucose metabolism, cell growth, and survival. It is particularly important in pancreatic β-cells, liver, and brain tissues, where it influences insulin secretion, gluconeogenesis, and neuronal function. Dysregulation of IRS2 is linked to metabolic disorders like type 2 diabetes, obesity, and certain cancers, making it a focal point for understanding disease mechanisms.
IRS2 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect IRS2 expression, localization, and post-translational modifications. These antibodies are typically raised against specific epitopes of the human or murine IRS2 protein and validated for specificity through knockout controls or siRNA silencing. Researchers rely on them to explore IRS2's involvement in insulin resistance, tumor progression, and neurodegenerative conditions. Commercial IRS2 antibodies vary in clonality (monoclonal/polyoclonal), host species, and conjugation tags, allowing flexibility in experimental design. Their development has advanced studies on therapeutic targeting of IRS2-related pathways for metabolic and oncological diseases.
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