| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Polymerase I and transcript release factor, Cavin-1, PTRF {ECO:0000312|EMBL:AAH661231} |
| Entrez GeneID | 284119 |
| WB Predicted band size | 43.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PTRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PTRF. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于PTRF (N-term)抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *"PTRF/Cavin-1 regulates caveolar formation and membrane plasticity in muscle cells"*
**作者**: Liu L. et al.
**摘要**: 该研究利用针对PTRF N端区域的抗体，通过免疫印迹和免疫荧光技术，揭示了PTRF在肌肉细胞中调控细胞膜穴样内陷(caveolae)形成的关键作用，并发现其缺失导致膜机械稳定性受损。
2. **文献名称**: *"Cavin family proteins and the assembly of caveolae"*
**作者**: Bastiani M. et al.
**摘要**: 研究通过PTRF N端特异性抗体证实，PTRF与Cavin家族其他成员协同参与细胞膜穴样结构的组装，并发现其N端结构域对膜结合功能至关重要。
3. **文献名称**: *"PTRF expression modulates tumorigenesis through lipid metabolism in breast cancer"*
**作者**: Mohan J. et al.
**摘要**: 使用PTRF N端抗体分析乳腺癌组织样本，发现PTRF通过调节脂代谢相关通路影响肿瘤生长，高表达与患者不良预后相关。
4. **文献名称**: *"Functional characterization of PTRF mutations in congenital generalized lipodystrophy"*
**作者**: Hasegawa T. et al.
**摘要**: 通过PTRF N端抗体检测患者突变模型，揭示PTRF基因突变导致细胞膜穴样结构异常及脂肪代谢紊乱的分子机制。
以上文献均涉及PTRF N端抗体的实验应用，涵盖功能研究、疾病模型及分子机制解析。
The PTRF (Polymerase I and Transcript Release Factor) antibody, specifically targeting the N-terminal region of the PTRF/Cavin-1 protein, is a tool for studying caveolae formation and cellular membrane dynamics. PTRF/Cavin-1. a member of the cavin family, is essential for biogenesis and stability of caveolae, specialized plasma membrane invaginations involved in lipid homeostasis, signal transduction, and endocytosis. The N-terminal domain of PTRF plays a critical role in its oligomerization and interaction with caveolin-1. a key structural protein of caveolae.
Research using PTRF (N-term) antibodies has elucidated its involvement in metabolic regulation, cancer biology, and genetic disorders. For instance, PTRF mutations are linked to congenital generalized lipodystrophy and muscular dystrophy, highlighting its role in adipocyte differentiation and membrane repair. The antibody is widely employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess PTRF expression levels, subcellular localization, and interactions in diverse tissues. Its specificity for the N-terminal region ensures detection of full-length, functional PTRF, distinguishing it from potential degradation products or isoforms. Validation via knockout cell lines or siRNA-mediated silencing is recommended to confirm target specificity in experimental models.
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