| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Folylpolyglutamate synthase, mitochondrial, Folylpoly-gamma-glutamate synthetase, FPGS, Tetrahydrofolylpolyglutamate synthase, Tetrahydrofolate synthase, FPGS |
| Entrez GeneID | 2356 |
| WB Predicted band size | 64.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FPGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the Central region of human FPGS. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FPGS抗体的3篇文献摘要示例(注：文献信息为模拟示例，实际需根据真实文献调整)：
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1. **文献名称**: *Development of a Monoclonal Antibody Targeting Human Folylpolyglutamate Synthase (FPGS) for Cancer Biomarker Analysis*
**作者**: Smith A, et al.
**摘要**: 本研究报道了一种针对人源FPGS蛋白的单克隆抗体的开发，验证了其特异性及在免疫组化中的应用。实验表明该抗体能有效检测肿瘤组织中FPGS的表达水平，为评估叶酸代谢相关化疗药物敏感性提供工具。
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2. **文献名称**: *FPGS Expression Modulates Methotrexate Resistance in Leukemia Cells: Insights from Antibody-Based Profiling*
**作者**: Chen L, et al.
**摘要**: 通过FPGS抗体检测白血病细胞系中的蛋白表达，发现FPGS低表达与甲氨蝶呤耐药性显著相关。研究提示FPGS抗体可作为预测化疗疗效的潜在生物标志物检测手段。
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3. **文献名称**: *Comparative Analysis of FPGS Isoforms in Human Tissues Using Species-Specific Antibodies*
**作者**: Tanaka K, et al.
**摘要**: 利用特异性抗体区分FPGS的不同剪接变体，揭示了其在正常组织与肿瘤中的分布差异，为研究FPGS功能多样性及靶向治疗策略提供新视角。
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(注：以上为模拟内容，实际文献需通过PubMed/Google Scholar等平台检索关键词“FPGS antibody”“folylpolyglutamate synthase antibody”获取。)
Folylpolyglutamate synthetase (FPGS) is a critical enzyme in folate metabolism, responsible for catalyzing the polyglutamylation of folate derivatives and certain antifolate drugs. This process involves the addition of glutamate residues, enhancing cellular retention and optimizing the activity of folate cofactors required for nucleotide synthesis and methylation reactions. FPGS plays a pivotal role in maintaining intracellular folate homeostasis, impacting DNA synthesis, repair, and epigenetic regulation.
FPGS antibodies are essential tools for studying the enzyme's expression, localization, and function in both physiological and pathological contexts. Research has highlighted FPGS's relevance in cancer biology, particularly in modulating the efficacy of antifolate chemotherapeutics (e.g., methotrexate). Tumor cells with low FPGS expression often exhibit resistance to these drugs due to reduced polyglutamylation and subsequent drug retention. Conversely, FPGS upregulation may influence folate dependency in proliferating cells.
Antibodies targeting FPGS enable detection via techniques like Western blot, immunohistochemistry, and immunofluorescence, aiding in biomarker studies and therapeutic response assessments. Dysregulated FPGS expression has been linked to folate deficiency disorders, neural tube defects, and oncogenesis. Recent studies also explore FPGS polymorphisms affecting enzyme activity and disease susceptibility. By elucidating FPGS's role in cellular metabolism and drug resistance, these antibodies support advancements in personalized oncology and folate-related disease management.
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