WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 1/10-1/50 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | OTU domain-containing protein 6B, DUBA-5, OTUD6B, DUBA5 |
Entrez GeneID | 51633 |
WB Predicted band size | 33.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This OTUD6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-262 amino acids from the C-terminal region of human OTUD6B. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与OTUD6B抗体相关的文献信息及摘要概括:
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1. **文献名称**:*OTUD6B suppresses tumorigenesis by regulating PTEN stability in colorectal cancer*
**作者**:Li Y, et al.
**摘要**:该研究揭示OTUD6B通过去泛素化调控PTEN蛋白稳定性,从而抑制结直肠癌进展。研究中使用特异性OTUD6B抗体进行免疫共沉淀(Co-IP)和Western blot实验,证实其与PTEN的相互作用及功能关联。
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2. **文献名称**:*OTUD6B regulates neurodevelopmental disorders through deubiquitination of TRAP1*
**作者**:Wang X, et al.
**摘要**:本文发现OTUD6B通过去泛素化线粒体蛋白TRAP1影响神经发育。研究通过OTUD6B抗体进行免疫荧光染色和蛋白质组学分析,证明其在神经元中的定位及与自闭症相关通路的联系。
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3. **文献名称**:*OTUD6B modulates innate immune responses by deubiquitinating MAVS*
**作者**:Chen Z, et al.
**摘要**:该研究报道OTUD6B通过调控MAVS蛋白的去泛素化增强抗病毒免疫反应。利用OTUD6B抗体进行免疫印迹和RNA干扰实验,证实其在固有免疫信号通路中的关键作用。
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注:上述文献为示例性内容,实际文献需通过PubMed或Google Scholar检索确认。若需具体文章,建议补充实验背景(如物种、研究领域)以便精准推荐。
OTUD6B (OTU domain-containing protein 6B) is a deubiquitinating enzyme (DUB) belonging to the ovarian tumor (OTU) subfamily of DUBs, which regulate protein stability and cellular signaling by removing ubiquitin chains from substrate proteins. OTUD6B is encoded by the OTUD6B gene located on human chromosome 8q24.3. It plays a role in diverse biological processes, including cell proliferation, apoptosis, and DNA damage repair, by modulating ubiquitination-dependent pathways. Studies suggest its involvement in ribosome biogenesis through interactions with nucleolar proteins, as well as potential links to neurodevelopmental disorders and cancers.
OTUD6B antibodies are essential tools for investigating its expression, localization, and molecular functions. These antibodies are commonly developed in hosts like rabbits or mice using immunogenic peptides derived from OTUD6B’s conserved regions. They are validated for applications such as Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. High-quality OTUD6B antibodies help identify its substrates and regulatory mechanisms, aiding research into diseases associated with dysregulated ubiquitination. Recent studies implicate OTUD6B mutations in intellectual disability syndromes, highlighting its significance in neurological development. However, its precise pathophysiological roles remain under active exploration, necessitating reliable antibody reagents for functional studies.
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