| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Enhancer of polycomb homolog 2, EPC-like, EPC2 |
| Entrez GeneID | 26122 |
| WB Predicted band size | 91.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This EPC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-233 amino acids from the N-terminal region of human EPC2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EPC2 (N-term)抗体的假设性参考文献示例(仅供参考，建议通过学术数据库核实具体文献)：
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1. **"EPC2 N-terminal antibody validates Polycomb complex interaction in gene silencing"**
*Author: Kim H, et al. (2013)*
**摘要**：本研究利用EPC2 N端特异性抗体，通过免疫共沉淀和Western blot技术，证实了EPC2与EZH2在Polycomb复合体中的直接相互作用，揭示了其在H3K27me3依赖的基因沉默中的作用。
2. **"Characterization of EPC2 isoforms in cancer using N-terminal specific antibodies"**
*Author: Smith JL, et al. (2015)*
**摘要**：通过开发针对EPC2 N端表位的抗体，作者在多种癌细胞系中检测到EPC2的不同剪接变体，并发现其异常表达与肿瘤侵袭性相关，为癌症表观遗传治疗提供潜在靶点。
3. **"Antibody-specific knockdown confirms EPC2 role in stem cell pluripotency"**
*Author: Jones R, et al. (2017)*
**摘要**：本文通过CRISPR-Cas9敲除结合EPC2 N-term抗体验证，证明EPC2在小鼠胚胎干细胞中通过调控PRC2复合体活性维持多能性，抗体特异性经敲除样本Western blot验证。
4. **"Epigenetic regulation by EPC2: Insights from ChIP-seq using a novel N-terminal antibody"**
*Author: Lee S, et al. (2018)*
**摘要**：利用新型EPC2 N端抗体进行ChIP-seq分析，揭示了EPC2在基因组中与特定发育基因启动子的结合模式，阐明其通过招募组蛋白修饰酶调控细胞分化的机制。
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**注意**：以上文献为示例性内容，实际文献需通过PubMed、Google Scholar等平台以关键词“EPC2 antibody”、“EPC2 N-term”或“EPC2 Polycomb”检索。若研究EPC2抗体的实验应用，建议关注抗体验证方法(如敲除/敲低实验)及功能研究相关论文。
The EPC2 (N-term) antibody is designed to target the N-terminal region of EPC2 (Eukaryotic PHD Finger Protein 2), also known as PHF19 or Polycomb-like Protein 3 (PCL3). EPC2 is a critical component of the Polycomb Repressive Complex 2 (PRC2), which mediates gene silencing through trimethylation of histone H3 on lysine 27 (H3K27me3), a hallmark of transcriptionally repressive chromatin. As a PRC2-associated protein, EPC2 facilitates the recruitment and stabilization of the complex at specific genomic loci, enhancing its enzymatic activity and chromatin-binding efficiency. The N-terminal domain of EPC2 is particularly important for interactions with other PRC2 core subunits, such as EZH2. SUZ12. and EED, as well as for binding to nucleic acids or chromatin-modifying cofactors.
EPC2 plays a role in stem cell maintenance, differentiation, and oncogenesis, with dysregulation linked to cancers and developmental disorders. The EPC2 (N-term) antibody is widely used in research to study PRC2-mediated epigenetic regulation, employing techniques like Western blotting, immunoprecipitation, and immunofluorescence. It helps elucidate EPC2’s spatial-temporal expression, post-translational modifications, and functional interplay within PRC2 or other chromatin-associated complexes. Studies using this antibody contribute to understanding diseases driven by aberrant Polycomb signaling and potential therapeutic strategies targeting epigenetic pathways.
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