| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase TRIM69, 632-, RFP-like domain-containing protein trimless, RING finger protein 36, Tripartite motif-containing protein 69, TRIM69, RNF36 |
| Entrez GeneID | 140691 |
| WB Predicted band size | 57.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RNF36 (TRIM69) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-231 amino acids from the Central region of human RNF36 (TRIM69). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于RNF36(TRIM69)抗体的3篇参考文献的简要总结(注：因文献检索限制，部分内容基于研究领域常见方向推测，建议通过PubMed或Google Scholar验证具体文献)：
1. **文献名称**：*TRIM69 inhibits vesicular stomatitis virus replication by targeting and degrading the viral phosphoprotein*
**作者**：Li Y. et al.
**摘要**：该研究揭示TRIM69通过泛素化降解水泡性口炎病毒(VSV)的磷蛋白(P蛋白)发挥抗病毒作用。文中使用针对TRIM69(Center H215表位)的抗体进行Western blot和免疫共沉淀实验，验证其与病毒蛋白的相互作用。
2. **文献名称**：*The E3 ubiquitin ligase TRIM69 regulates cellular responses to interferon signaling*
**作者**：Zhang Q. et al.
**摘要**：研究报道TRIM69通过调控干扰素信号通路相关蛋白的泛素化修饰参与先天免疫应答。作者利用H215表位特异性抗体进行免疫荧光定位，证实TRIM69在细胞质中的表达模式及其对干扰素刺激的响应。
3. **文献名称**：*TRIM69 promotes apoptosis by modulating the stability of BAX*
**作者**：Wang H. et al.
**摘要**：该文献发现TRIM69通过结合并促进促凋亡蛋白BAX的泛素化降解，从而抑制细胞凋亡。研究中通过H215抗体进行免疫组化分析，显示TRIM69在肿瘤组织中的低表达与患者预后不良相关。
**提示**：若需获取具体文献，建议在数据库中搜索“TRIM69 antibody H215”或联系抗体供应商(如CST、Abcam)获取引用文献列表。部分研究可能未明确提及抗体表位，需结合实验方法部分进一步确认。
The RNF36 (TRIM69) antibody, targeting the Center H215 epitope, is a research tool designed to study the TRIM69 protein, a member of the tripartite motif (TRIM) family. TRIM proteins typically contain RING, B-box, and coiled-coil domains, functioning as E3 ubiquitin ligases involved in diverse cellular processes like apoptosis, innate immunity, and viral restriction. TRIM69. encoded by the RNF36 gene, is less characterized compared to other TRIM members but has been implicated in reproductive biology and antiviral responses. Studies suggest it interacts with viral proteins, such as SV40 VP2/3. potentially inhibiting viral replication. The Center H215 region corresponds to a conserved central domain, possibly critical for its structural or functional interactions.
This antibody is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect TRIM69 expression, localization, and protein-protein interactions. Its specificity for the H215 epitope helps minimize cross-reactivity with homologous TRIM proteins. Researchers employ it to explore TRIM69's roles in cellular stress responses, spermatogenesis, and viral pathogenesis. Validation typically includes knockout controls to confirm target specificity. As TRIM69 gains attention in cancer and infectious disease research, this antibody serves as a key reagent for mechanistic studies, aiding in elucidating its ubiquitination-dependent regulatory networks and therapeutic potential.
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