Rabbit Polyclonal RPL4(N-Term) Antibody

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     All lanes : Anti-RPL4 Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: NCI-H929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling RPL4 with P33834 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG  secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing U-2OS cells stained with P33834 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33834,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The RPL4 (N-Term) antibody is designed to target the N-terminal region of the Ribosomal Protein L4 (RPL4), a component of the 60S ribosomal subunit. RPL4 is essential for ribosome assembly and protein synthesis, playing a critical role in translating mRNA into functional proteins. As part of the large ribosomal subunit, RPL4 contributes to the structural stability of the ribosome and facilitates interactions during translation.

This antibody is commonly used in research to study ribosomal biogenesis, cellular stress responses, and diseases linked to ribosomal dysfunction, such as ribosomopathies or cancers. By specifically recognizing the N-terminal epitope, it enables detection of full-length RPL4 in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers also utilize it to investigate post-translational modifications or subcellular localization of RPL4 under varying physiological conditions.

Dysregulation of RPL4 has been implicated in developmental disorders and tumorigenesis, making this antibody a valuable tool for exploring molecular mechanisms underlying these conditions. Validation typically includes specificity confirmation via knockout controls or peptide blocking assays to ensure minimal cross-reactivity. Its applications extend to both basic and clinical research, bridging insights into ribosomal biology and disease pathology.