| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, hBravo, NgCAM-related cell adhesion molecule, Ng-CAM-related, NRCAM, KIAA0343 |
| Entrez GeneID | 4897 |
| WB Predicted band size | 143.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NRCAM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 30-62 amino acids of mouse NRCAM. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NRCAM (N-Term)抗体的参考文献示例(内容基于学术文献常见主题概括，非真实文献)：
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1. **"Characterization of a Novel N-Terminal Specific NRCAM Antibody in Neuronal Development"**
*作者：Smith A, et al.*
摘要：本研究开发并验证了一种针对NRCAM N末端结构域的多克隆抗体，证实其特异性通过Western blot和免疫组化分析。该抗体成功用于检测小鼠大脑皮层神经元中NRCAM的定位，并揭示其在轴突导向中的作用。
2. **"NRCAM Expression in Glioblastoma: Role of N-Terminal Epitope Recognition"**
*作者：Chen L, et al.*
摘要：通过使用NRCAM N端抗体，研究发现胶质母细胞瘤细胞中NRCAM蛋白表达显著上调，并与肿瘤侵袭性相关。抗体特异性通过siRNA敲低实验验证，支持其在临床病理检测中的应用潜力。
3. **"Structural Insights into NRCAM-Mediated Synaptic Adhesion Using Domain-Specific Antibodies"**
*作者：Kuroda Y, et al.*
摘要：文章利用N末端抗体解析NRCAM在突触形成中的分子机制。免疫电镜显示NRCAM的N端结构域与神经配体结合，调控突触可塑性，为神经退行性疾病研究提供工具。
4. **"Validation of N-Terminal NRCAM Antibody for Flow Cytometry in Neural Crest Cell Analysis"**
*作者：Rodriguez M, et al.*
摘要：研究优化了NRCAM N端抗体在流式细胞术中的应用，证明其可用于活体神经嵴细胞的表面标记分选，并揭示NRCAM在胚胎发育中的动态表达模式。
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注：以上为模拟示例，实际文献需通过PubMed或学术数据库检索确认。
The NRCAM (N-Term) antibody is designed to target the N-terminal region of the Neuronal Cell Adhesion Molecule (NRCAM), a member of the L1 immunoglobulin superfamily involved in neural development, axon guidance, and synaptic plasticity. NRCAM plays critical roles in cell-cell adhesion, neuronal migration, and signal transduction, primarily through interactions with extracellular matrix components and other cell surface receptors. The N-terminal domain is essential for mediating these interactions, influencing processes like neurite outgrowth and network formation.
Antibodies against the N-terminal region are widely used in neuroscience research to study NRCAM expression, localization, and function in both physiological and pathological contexts. They are employed in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize protein distribution in brain tissues, cultured neurons, or transfected cell lines. Specificity for the N-terminal domain ensures recognition of full-length NRCAM, aiding in distinguishing it from proteolytic fragments or isoforms.
Research involving NRCAM (N-Term) antibodies has contributed to understanding neurodevelopmental disorders, neurodegenerative diseases, and cancer, where dysregulated NRCAM expression is implicated. These tools are validated for use in human, mouse, and rat samples, supporting cross-species comparative studies. Proper controls, such as knockout validation or peptide blocking, are recommended to confirm antibody specificity in experimental setups.
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