Rabbit Polyclonal RNF135(C-term) Antibody

The RNF135 (C-term) antibody is designed to target the C-terminal region of the RNF135 protein, a member of the E3 ubiquitin ligase family involved in ubiquitination, a post-translational modification critical for protein degradation, signaling, and cellular regulation. RNF135. also known as RING finger protein 135. plays roles in diverse processes, including antiviral immune responses, DNA repair, and tumorigenesis. Studies link it to diseases like glioblastoma and neurodevelopmental disorders, with mutations reported in syndromes such as Fontaine progeroid syndrome. The C-terminal-specific antibody is commonly used to detect full-length RNF135 or its isoforms in applications like Western blotting, immunofluorescence, and immunoprecipitation. Its specificity for the C-terminus helps distinguish RNF135 from related E3 ligases, aiding research into its functional domains, interaction partners, and regulatory mechanisms. Validation typically includes testing in knockout models or siRNA-treated cells to confirm target selectivity. Researchers employ this antibody to explore RNF135's role in ubiquitin-mediated pathways, its involvement in disease mechanisms, and potential therapeutic targeting. Proper controls are essential to ensure accurate interpretation, given possible cross-reactivity or isoform variability.