| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear receptor-interacting protein 1, Nuclear factor RIP140, Receptor-interacting protein 140, NRIP1 |
| Entrez GeneID | 8204 |
| WB Predicted band size | 126.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RIP140 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-613 amino acids from the Central region of human RIP140. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于RIP140抗体的参考文献示例(内容为虚构，仅作格式参考)：
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1. **文献名称**: *"Development and validation of a RIP140-specific monoclonal antibody for chromatin immunoprecipitation assays"*
**作者**: Müller, S. et al.
**摘要**: 本研究开发了一种高特异性的RIP140单克隆抗体，并通过Western blot和免疫荧光验证其特异性。该抗体成功应用于染色质免疫沉淀(ChIP)实验，揭示了RIP140在雌激素受体靶基因启动子上的动态招募机制。
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2. **文献名称**: *"RIP140 antibody-based profiling reveals its suppressive role in adipocyte differentiation"*
**作者**: Wang, Y. & Tang, L.
**摘要**: 通过使用商业化RIP140多克隆抗体，研究团队发现RIP140在脂肪细胞分化过程中表达下调，并证实其通过抑制PPARγ活性调控脂代谢相关基因的转录。
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3. **文献名称**: *"A novel RIP140 antibody uncovers its cytoplasmic localization in neuronal cells"*
**作者**: Gomez, R. et al.
**摘要**: 本文报道了一种新型RIP140抗体，意外发现RIP140在神经元细胞质中富集，并通过免疫电镜证实其与线粒体的共定位，提示其可能参与能量代谢调控。
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4. **文献名称**: *"RIP140 antibody cross-reactivity analysis in murine and human tissues"*
**作者**: Chen, X. et al.
**摘要**: 系统性评估了多种RIP140抗体在小鼠和人类组织中的交叉反应性，为不同模型系统中抗体的选择提供了实验依据，并揭示了物种间RIP140表达差异。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“RIP140 antibody”，筛选应用或开发该抗体的实验性研究(如Western blot、IHC等)。
Receptor-interacting protein 140 (RIP140), also known as NRIP1 (nuclear receptor-interacting protein 1), is a transcriptional coregulator that interacts with nuclear receptors to modulate gene expression. It plays dual roles in transcriptional activation and repression, depending on cellular context and post-translational modifications. RIP140 is involved in diverse physiological processes, including metabolism, reproduction, and cancer progression, by regulating genes linked to energy homeostasis, steroidogenesis, and cell proliferation.
RIP140 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are commonly used in techniques such as Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunohistochemistry (IHC). Polyclonal and monoclonal RIP140 antibodies are developed against specific epitopes, often validated using knockout cell lines or tissues to ensure specificity.
Research applications include investigating RIP140's role in metabolic disorders (e.g., obesity, insulin resistance) and cancer, where it may act as an oncogene or tumor suppressor. For instance, RIP140 overexpression in breast and ovarian cancers correlates with disease progression. Commercial RIP140 antibodies are available from suppliers like Abcam, Santa Cruz Biotechnology, and Cell Signaling Technology, often accompanied by validation data. Proper controls, such as siRNA knockdown or RIP140-deficient samples, are critical for accurate experimental interpretation. These antibodies continue to support mechanistic studies of nuclear receptor signaling and transcriptional regulation.
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