| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2B 1A; H2B; H2B histone family; H2B2f; H2Ba; H2Bf; HIST2H2BF; histone H2B; histone H2B type 1; Histone H2B type 2-F;;Acetyl-Histone H2B (K20) |
| WB Predicted band size | Calculated MW: 14 kDa ; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H2B around the acetylation site of K20 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ERCC5抗体的示例参考文献(内容为模拟，非真实文献)：
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1. **标题**: "ERCC5/XPG Protein Expression in Human Cancers: A Biomarker for DNA Repair Capacity"
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot和免疫组化技术，利用特异性ERCC5抗体分析了多种癌症组织中XPG蛋白的表达水平，发现ERCC5低表达与化疗耐药性及患者生存率降低显著相关。
2. **标题**: "Development of a Monoclonal Antibody for Human ERCC5 and Its Application in DNA Damage Studies"
**作者**: Tanaka K, et al.
**摘要**: 文章报道了一种高特异性小鼠单克隆ERCC5抗体的制备，验证了其在免疫荧光和免疫沉淀实验中的应用，证实ERCC5在紫外线诱导的DNA损伤修复中的动态定位。
3. **标题**: "ERCC5 Genetic Variants and Protein Expression in Melanoma Progression"
**作者**: Lee J, et al.
**摘要**: 通过免疫组化结合ERCC5抗体检测黑色素瘤样本，发现ERCC5表达水平与肿瘤侵袭性相关，并揭示了其通过调控核苷酸切除修复通路影响基因组稳定性。
4. **标题**: "Functional Characterization of ERCC5 Antibodies in Knockout Cell Models"
**作者**: Garcia-Saez I, et al.
**摘要**: 研究利用CRISPR/Cas9敲除细胞系，验证了多种商业化ERCC5抗体的特异性，强调了抗体选择对准确检测XPG蛋白表达及功能研究的重要性。
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注：以上文献为示例，实际引用时需查询真实数据库(如PubMed)获取具体信息。
The ERCC5 antibody is a crucial tool in studying the excision repair cross-complementation group 5 (ERCC5) protein, also known as XPG. ERCC5 is a conserved endonuclease essential for nucleotide excision repair (NER), a pathway that removes bulky DNA lesions caused by UV radiation, chemical mutagens, or oxidative stress. Structurally, XPG contains a helicase-like domain and a catalytic nuclease domain, enabling its role in cleaving damaged DNA strands during NER. Beyond DNA repair, ERCC5 interacts with proteins involved in transcription and genome stability, linking it to aging, cancer, and neurodegeneration.
Antibodies targeting ERCC5 are widely used to investigate its expression, localization, and functional roles in cellular models and clinical samples. They are critical for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess XPG levels in tissues or cell lines, particularly in contexts like chemotherapy resistance or DNA damage response studies. Mutations in ERCC5 are associated with xeroderma pigmentosum (XP-G), Cockayne syndrome, and increased cancer susceptibility, making these antibodies valuable for diagnosing XP variants or studying genotype-phenotype correlations. Commercially available ERCC5 antibodies are typically validated using knockout cell lines or recombinant proteins to ensure specificity. Researchers also utilize them to explore ERCC5's potential as a biomarker for predicting therapeutic outcomes in cancers treated with DNA-damaging agents.
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