| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pleckstrin homology domain-containing family F member 1, PH domain-containing family F member 1, Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains, Apoptosis-inducing protein, PH and FYVE domain-containing protein 1, Phafin-1, Zinc finger FYVE domain-containing protein 15, PLEKHF1, APPD, LAPF, ZFYVE15 |
| Entrez GeneID | 79156 |
| WB Predicted band size | 31.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PLEKHF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human PLEKHF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PLEKHF1 (N-term)抗体的3篇参考文献，按文献名称、作者及摘要内容简要概括：
1. **文献名称**：*"Characterization of a Novel Monoclonal Antibody Targeting the N-Terminal Domain of PLEKHF1 for Functional Studies in Endosomal Trafficking"*
**作者**：Smith A, et al.
**摘要**：该研究开发了一种针对PLEKHF1蛋白N端的新型单克隆抗体，验证了其在免疫印迹(Western blot)和免疫荧光(IF)中的特异性，并发现PLEKHF1通过其N端结构域参与调控内体膜运输过程。
2. **文献名称**：*"PLEKHF1 Antibody-Based Detection of Oxidative Stress-Induced Protein Modifications in Neurodegenerative Disease Models"*
**作者**：Chen L, et al.
**摘要**：研究利用N端特异性PLEKHF1抗体，发现氧化应激条件下PLEKHF1在帕金森病模型中的表达异常，提示其可能通过N端介导的蛋白相互作用参与神经退行性病变的分子机制。
3. **文献名称**：*"Development and Validation of a Polyclonal Antibody Against the N-Terminal Region of Human PLEKHF1 for Apoptosis Assays"*
**作者**：Garcia R, et al.
**摘要**：本文报道了一种针对人源PLEKHF1 N端的多克隆抗体的制备与验证，证实其在流式细胞术和免疫组化中的适用性，并发现PLEKHF1的N端区域在细胞凋亡信号通路中具有调控作用。
4. **文献名称**：*"Interactome Analysis of PLEKHF1 Using N-Terminal Specific Antibodies Reveals Novel Binding Partners in Cancer Cell Migration"*
**作者**：Wang Y, et al.
**摘要**：通过N端抗体免疫沉淀结合质谱分析，鉴定了PLEKHF1在乳腺癌细胞中的新型相互作用蛋白，揭示了其N端结构域在肿瘤细胞迁移和侵袭中的潜在功能。
(注：上述文献为虚拟示例，实际引用需查询具体数据库如PubMed、Google Scholar等获取真实信息。)
The PLEKHF1 (N-term) antibody is a selective immunological tool designed to target the N-terminal region of the human PLEKHF1 (Pleckstrin Homology and FYVE Domain-Containing Protein 1). PLEKHF1 is a multifunctional protein implicated in intracellular vesicle trafficking, membrane dynamics, and endosomal-lysosomal pathways. Its structure includes a pleckstrin homology (PH) domain and a FYVE domain, both critical for lipid binding and membrane association. The N-terminal region, where this antibody binds, is thought to play a role in protein-protein interactions and subcellular localization.
Research using this antibody has helped elucidate PLEKHF1's involvement in autophagy, apoptosis, and cellular stress responses. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study expression patterns, tissue distribution, and molecular interactions of PLEKHF1. Dysregulation of PLEKHF1 has been linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, making this antibody valuable for both basic research and translational studies. Validation data typically include reactivity in human samples and specificity confirmed by knockout controls. Its applications contribute to understanding PLEKHF1's role in cellular homeostasis and disease pathology.
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