Rabbit Polyclonal NANOG(N-term) Antibody

+ +

•  

   
     All lanes : Anti-NANOG Antibody (N-term) at 1:1000 dilution Lane 1: A2780 whole cell lysate Lane 2: OVCAR3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#P33758) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#P33758). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).    

•  

   
     Fluorescent confocal image of SY5Y cells stained with P33758 NANOG (N-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with P33758 NANOG (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.    

•  

   
     NANOG Antibody (N-term) (Cat. #P33758) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.    

•  

   
     Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term)(Cat.#P33758), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.    

The NANOG (N-term) antibody is a specialized tool used to detect the N-terminal region of the NANOG protein, a key transcription factor critical for maintaining pluripotency in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). NANOG, named after the Celtic myth "Tír na nÓg" ("Land of the Young"), plays a central role in self-renewal by suppressing differentiation signals and sustaining the undifferentiated state of stem cells. It functions in a regulatory network with OCT4 and SOX2. forming a core circuitry essential for pluripotency.

The antibody is commonly employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study NANOG expression and localization. Its specificity for the N-terminal domain ensures recognition of full-length, functional NANOG, distinguishing it from potential truncated isoforms or degradation products. Researchers use it to explore NANOG's roles in early embryonic development, cellular reprogramming, and cancer biology, where aberrant NANOG expression is linked to tumorigenesis and metastasis.

Validated in various applications and species (e.g., human, mouse), the NANOG (N-term) antibody is crucial for advancing stem cell research, regenerative medicine, and oncological studies. Its reliability depends on proper controls, such as knockout cell lines, to confirm target specificity.