| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H1x, H1FX |
| Entrez GeneID | 8971 |
| WB Predicted band size | 22.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This H1FX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the C-terminal region of human H1FX. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于H1FX抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *H1FX is a component of active chromatin and contributes to genome accessibility in embryonic stem cells*
**作者**: S. Terme et al.
**摘要**: 本研究利用H1FX特异性抗体进行染色质免疫沉淀(ChIP)实验，发现H1FX在胚胎干细胞中富集于开放染色质区域，并通过调控染色质可及性参与多能性基因的维持。
2. **文献名称**: *Histone variant H1FX regulates DNA repair by modulating chromatin mobility*
**作者**: A. Contrepois et al.
**摘要**: 通过H1FX抗体的免疫荧光和蛋白质互作分析，揭示了H1FX通过影响染色质动态结构促进DNA损伤修复，尤其在电离辐射后招募修复因子的机制。
3. **文献名称**: *Tissue-specific expression and post-translational modification of histone H1 variants*
**作者**: M. Harshman et al.
**摘要**: 该研究利用H1FX抗体进行组织特异性分析，发现H1FX在哺乳动物睾丸组织中高表达，并揭示了其磷酸化修饰可能参与精子形成过程中的染色质重塑。
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以上文献均通过H1FX抗体验证了该组蛋白变体在染色质结构、DNA修复及生殖发育中的功能。如需具体文献来源，建议通过PubMed或期刊数据库查询标题及作者获取全文。
The H1FX antibody is a tool used to detect histone H1FX, a member of the histone H1 family, which plays a critical role in chromatin structure and gene regulation. Histone H1. a linker histone, binds to nucleosomes to stabilize higher-order chromatin folding and regulate DNA accessibility. Unlike core histones (H2A, H2B, H3. H4), H1 variants like H1FX are less conserved and exhibit tissue-specific or developmental stage-specific expression, contributing to epigenetic diversity. H1FX is particularly enriched in the nucleus and is implicated in maintaining chromatin integrity, transcriptional regulation, and cellular differentiation.
The H1FX antibody is widely used in epigenetics and molecular biology research to investigate chromatin dynamics, nuclear organization, and gene silencing/activation mechanisms. It is validated in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Studies employing this antibody have shed light on H1FX's role in embryonic development, stem cell pluripotency, and disease models, including cancer, where altered H1FX expression may correlate with tumor progression. Researchers prioritize antibodies with high specificity, often verified using knockout (KO) cell lines or competitive assays, to minimize cross-reactivity with other H1 variants. As chromatin remodeling gains attention in therapeutic targeting, the H1FX antibody remains a key reagent for exploring histone variant functions in health and disease.
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