| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Notch-regulated ankyrin repeat-containing protein, NRARP |
| Entrez GeneID | 441478 |
| WB Predicted band size | 12.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NRARP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human NRARP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NRARP抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
1. **文献名称**: *"NRARP regulates Notch signaling to maintain endothelial cell fate in vascular development"*
**作者**: Liu Z, et al.
**摘要**: 研究斑马鱼模型中发现NRARP通过稳定Notch信号通路维持内皮细胞命运，利用特异性NRARP抗体进行免疫荧光定位及功能缺失实验，证明其在血管分支形成中的关键作用。
2. **文献名称**: *"Dynamic expression of NRARP in Wnt-driven intestinal stem cell differentiation"*
**作者**: Garcia-Lopez J, et al.
**摘要**: 通过小鼠肠道类器官模型，揭示NRARP在Wnt/Notch信号交互中的调控作用，使用NRARP抗体进行Western blot和免疫组化，证实其表达与干细胞分化状态的关联。
3. **文献名称**: *"NRARP as a biomarker in T-cell acute lymphoblastic leukemia: Antibody-based detection and clinical implications"*
**作者**: Chen H, et al.
**摘要**: 分析人类T-ALL样本中NRARP蛋白的过表达与Notch信号激活的关系，开发高灵敏度NRARP抗体用于流式细胞术和病理诊断，提示其作为潜在治疗靶点。
4. **文献名称**: *"NRARP interacts with Dll4 to modulate tip cell selection in angiogenesis"*
**作者**: Benedito R, et al.
**摘要**: 结合体外细胞共培养和小鼠视网膜模型，通过NRARP抗体的共免疫沉淀实验，阐明NRARP与Dll4的相互作用如何精细调控内皮尖端细胞竞争及血管分支模式。
(注：以上文献为虚拟示例，实际引用需根据真实研究补充。)
The NRARP (Notch-Regulated Ankyrin Repeat Protein) antibody is a tool used to study the role of NRARP, a small adaptor protein regulated by the Notch signaling pathway. NRARP contains two conserved ankyrin repeat domains that mediate protein-protein interactions, enabling its function in modulating both Notch and Wnt/β-catenin signaling cascades. It is expressed in various tissues, including vascular endothelium and hematopoietic cells, where it contributes to developmental processes, stem cell maintenance, and tissue homeostasis.
NRARP acts as a feedback regulator of Notch signaling by promoting the degradation of active Notch intracellular domain (NICD), thereby fine-tuning pathway activity. It also interacts with components of the Wnt pathway, linking crosstalk between these critical developmental pathways. Dysregulation of NRARP has been implicated in pathologies such as T-cell leukemia, angiogenesis defects, and cancer progression.
The NRARP antibody is widely used in research to detect protein expression, localization, and interaction partners via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Its applications extend to studying Notch/Wnt-related diseases, drug discovery, and genetic models (e.g., zebrafish, mice). This antibody serves as a key reagent for unraveling NRARP's dual regulatory roles and its therapeutic potential in signaling-driven disorders.
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