Rabbit Polyclonal PHF5A Antibody

The PHF5A (PHD Finger Protein 5A) antibody is a tool used to study the PHF5A protein, a conserved eukaryotic protein involved in transcriptional regulation and RNA splicing. PHF5A contains a PHD (plant homeodomain) zinc finger motif, which typically mediates interactions with histone modifications or other chromatin-associated proteins. It is a component of the SF3b complex within the spliceosome, playing a critical role in pre-mRNA splicing by stabilizing spliceosome assembly and ensuring splicing fidelity. PHF5A also interacts with chromatin modifiers, linking splicing to epigenetic regulation. Dysregulation of PHF5A is implicated in cancer, particularly in hematological malignancies (e.g., acute myeloid leukemia) and solid tumors, where its overexpression or mutations correlate with aberrant splicing, genomic instability, and oncogenic signaling. PHF5A antibodies are widely used in research to investigate its expression, localization, and molecular interactions via techniques like Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. These antibodies are essential for elucidating PHF5A's role in splicing mechanisms, cellular differentiation, and cancer progression. Validation of PHF5A antibodies often includes knockout cell lines to confirm specificity. Their diagnostic and therapeutic potential is under exploration, particularly in cancers with splicing-related vulnerabilities.