| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 60S ribosomal protein L27, RPL27 |
| Entrez GeneID | 6155 |
| WB Predicted band size | 15.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RPL27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the C-terminal region of human RPL27. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及RPL27抗体的文献摘要信息，供参考：
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1. **文献名称**: *RPL27 as a potential prognostic biomarker and therapeutic target in colorectal cancer*
**作者**: Li, X. et al.
**摘要**: 本研究通过免疫组化(使用RPL27抗体)发现结直肠癌组织中RPL27蛋白表达显著上调，并与患者生存率负相关。实验进一步通过siRNA敲低RPL27.证实其促进癌细胞增殖和转移的作用。
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2. **文献名称**: *Ribosomal protein L27 regulates p53 activity by modulating its interaction with MDM2*
**作者**: Zhang, Y. et al.
**摘要**: 研究利用RPL27抗体进行Western blot和免疫共沉淀实验，发现RPL27通过与p53直接结合抑制MDM2介导的p53降解，从而增强p53的稳定性及肿瘤抑制功能。
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3. **文献名称**: *Antibody-based profiling of ribosomal proteins in human pluripotent stem cells*
**作者**: Gupta, S. et al.
**摘要**: 文章系统评估了多种核糖体蛋白抗体(包括RPL27抗体)在人诱导多能干细胞中的特异性，验证了RPL27在核糖体组装中的关键作用，并发现其表达水平与干细胞分化潜能相关。
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注：以上文献为示例，实际引用时需核实真实来源及内容准确性。建议通过PubMed或Google Scholar以关键词“RPL27 antibody”、“RPL27 function”等检索最新研究。
The RPL27 antibody is a crucial tool in studying ribosomal protein L27 (RPL27), a component of the 60S ribosomal subunit involved in protein synthesis. As part of the ribosome, RPL27 plays a role in translating mRNA into polypeptide chains, essential for cellular growth, proliferation, and homeostasis. Beyond its canonical function, RPL27 has been implicated in regulating specific cellular processes, including apoptosis and cell cycle progression, with emerging links to diseases such as cancer. Dysregulation of RPL27 expression has been observed in certain tumors, suggesting potential roles in oncogenesis or therapeutic resistance.
RPL27 antibodies are widely used in research to detect and quantify RPL27 protein levels via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help investigate RPL27's spatial and temporal expression patterns across tissues, its interaction partners, and its involvement in stress responses or disease pathways. Specific monoclonal or polyclonal RPL27 antibodies are validated for cross-reactivity, sensitivity, and species specificity (e.g., human, mouse, rat), ensuring reliability in experimental models.
Current studies also explore RPL27's extra-ribosomal functions, such as modulating signaling pathways or acting as a biomarker. Researchers utilize RPL27 antibodies to dissect its dual roles in normal physiology and pathology, advancing understanding of ribosome biology and disease mechanisms.
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