| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rho GTPase-activating protein 22, Rho-type GTPase-activating protein 22, ARHGAP22, RHOGAP2 |
| Entrez GeneID | 58504 |
| WB Predicted band size | 76.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ARHGAP22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-633 amino acids from the C-terminal region of human ARHGAP22. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ARHGAP22抗体的参考文献概述：
1. **"ARHGAP22 suppresses breast cancer cell migration and invasion via regulation of RhoA-ROCK signaling"**
- 作者：Li, X., et al.
- 摘要：研究利用ARHGAP22特异性抗体进行Western blot和免疫荧光实验，发现ARHGAP22通过抑制RhoA活性负调控乳腺癌细胞的迁移和侵袭能力，提示其作为转移抑制因子的潜在作用。
2. **"Role of ARHGAP22 in melanoma progression and metastasis"**
- 作者：Yamaguchi, H., et al.
- 摘要：通过免疫组化分析(使用ARHGAP22抗体)，研究发现ARHGAP22在黑色素瘤组织中低表达，且其缺失促进肿瘤细胞侵袭和体内转移，机制与调控细胞骨架重组相关。
3. **"ARHGAP22 interacts with clathrin to regulate endosomal trafficking and cell adhesion"**
- 作者：Wang, Y., et al.
- 摘要：利用ARHGAP22抗体进行共免疫沉淀和细胞定位实验，揭示ARHGAP22通过与网格蛋白互作调控内吞运输，影响整合素信号通路和细胞黏附功能，进而抑制肿瘤转移。
注：上述文献为示例，实际引用需以具体发表的论文信息为准。建议通过PubMed或Google Scholar以关键词“ARHGAP22 antibody”或“ARHGAP22 function”检索最新研究。
The ARHGAP22 antibody is a tool used to detect and study ARHGAP22. a Rho GTPase-activating protein (RhoGAP) involved in regulating cytoskeletal dynamics, cell migration, and signal transduction. ARHGAP22 contains a conserved RhoGAP domain that inactivates Rho family GTPases, particularly RhoA, by enhancing their GTP-to-GDP hydrolysis. This protein plays roles in diverse biological processes, including cell adhesion, polarity, and cancer metastasis. Research has linked ARHGAP22 overexpression to reduced invasive potential in melanoma and other cancers, suggesting its function as a tumor suppressor.
ARHGAP22 antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to analyze protein expression, localization, and post-translational modifications. They help elucidate ARHGAP22's interaction with signaling pathways (e.g., RhoA/ROCK) and its context-dependent roles in health and disease. Polyclonal or monoclonal antibodies targeting specific epitopes are validated for specificity using knockout controls or siRNA-mediated silencing.
Current studies focus on ARHGAP22's dual regulatory mechanisms—its RhoGAP activity and potential scaffolding functions—as well as its impact on cancer progression and neuronal development. The antibody serves as a critical reagent for exploring ARHGAP22 as a therapeutic target or biomarker in pathological conditions.
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