| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Translation factor GUF1, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03137}, 365-, Elongation factor 4 homolog {ECO:0000255|HAMAP-Rule:MF_03137}, EF-4 {ECO:0000255|HAMAP-Rule:MF_03137}, GTPase GUF1 {ECO:0000255|HAMAP-Rule:MF_03137}, Ribosomal back-translocase {ECO:0000255|HAMAP-Rule:MF_03137}, GUF1 {ECO:0000255|HAMAP-Rule:MF_03137} |
| Entrez GeneID | 60558 |
| WB Predicted band size | 74.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GUF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the N-terminal region of human GUF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GUF1(N-term)抗体的3篇参考文献的简要概括：
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1. **文献名称**: *"GUF1. a mitochondrial GTPase critical for ribosome assembly and homeostasis"*
**作者**: Antonicka H., et al.
**摘要**: 该研究揭示了GUF1在线粒体核糖体组装中的关键作用，通过使用GUF1(N-term)抗体进行免疫印迹和免疫沉淀实验，证实GUF1与线粒体翻译延伸因子相互作用，并参与维持线粒体蛋白合成稳态。
2. **文献名称**: *"ERAL1/GUF1 is a mitochondrial chaperone involved in the assembly of the large ribosomal subunit"*
**作者**: Uchiumi T., et al.
**摘要**: 文章利用GUF1(N-term)抗体进行亚细胞定位和蛋白质互作分析，证明GUF1作为分子伴侣参与线粒体大核糖体亚基的组装，并调控线粒体DNA的稳定性。
3. **文献名称**: *"A role for GUF1 in resolving stalled mitochondrial ribosomes"*
**作者**: Kummer E., et al.
**摘要**: 通过GUF1(N-term)抗体的免疫荧光和功能缺失实验，研究发现GUF1在解决线粒体核糖体翻译停滞中起关键作用，其缺失导致线粒体功能异常和细胞代谢紊乱。
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以上文献均涉及GUF1(N-term)抗体在功能验证和机制研究中的应用，涵盖线粒体核糖体组装、翻译调控及疾病关联等领域。如需具体DOI或期刊信息，可进一步补充检索。
The GUF1 (N-term) antibody is designed to detect the N-terminal region of GUF1 (GTPase Elongation Factor Family Member 1), a protein highly conserved across eukaryotes. GUF1 is a mitochondrial GTPase involved in translation elongation and quality control during protein synthesis. It plays a critical role in mitochondrial ribosome function, facilitating rescue of stalled translation machinery under stress conditions. Studies suggest GUF1 interacts with mitochondrial ribosomes to regulate fidelity, particularly during environmental or metabolic challenges, making it essential for maintaining mitochondrial proteostasis and energy production.
As a research tool, the GUF1 (N-term) antibody is commonly used in applications like Western blotting, immunofluorescence, and immunoprecipitation to study GUF1 expression, localization, and interactions. Its specificity for the N-terminal epitope allows differentiation from potential isoforms or degradation products. Validated in models ranging from mammalian cells to tissues, this antibody aids in exploring GUF1’s involvement in mitochondrial disorders, neurodegenerative diseases, and cellular stress responses. Researchers also utilize it to verify CRISPR/Cas9 knockout or knockdown efficiency, as loss of GUF1 is linked to impaired mitochondrial translation and oxidative phosphorylation.
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