| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3, NDUFAF3, C3orf60 |
| Entrez GeneID | 25915 |
| WB Predicted band size | 20.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NDUF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human NDUF3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于NDUF3(N-term)抗体相关研究的**模拟参考文献示例**(非真实文献，仅供格式参考)：
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1. **文献名称**: *"Characterization of a Novel NDUF3-Specific Antibody for Detecting Mitochondrial Complex I Dysfunction in Parkinson's Disease Models"*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对NDUF3 N端表位的高特异性抗体，用于检测帕金森病细胞模型中线粒体复合物I的组装缺陷。抗体通过Western blot和免疫荧光验证，证明其在疾病模型中可敏感检测NDUF3蛋白水平下降。
2. **文献名称**: *"NDUFF3 as a Biomarker in Colorectal Cancer: Validation of an N-Terminal Antibody for Immunohistochemical Staining"*
**作者**: Lee J, et al.
**摘要**: 文章验证了一种商业化NDUF3(N-term)抗体在结直肠癌组织中的适用性，发现NDUF3表达降低与线粒体代谢异常和患者预后不良相关，为癌症代谢研究提供了工具。
3. **文献名称**: *"Antibody-Based Profiling of Mitochondrial Complex I Subunits in Leigh Syndrome Patients"*
**作者**: García-Ruiz C, et al.
**摘要**: 利用NDUF3(N-term)抗体分析Leigh综合征患者成纤维细胞，发现复合物I亚基组装异常，表明该抗体可用于线粒体疾病中复合物I功能缺陷的诊断。
4. **文献名称**: *"Optimization of NDUF3 Antibody for Cross-Species Reactivity in Zebrafish Models"*
**作者**: Chen H, et al.
**摘要**: 研究优化了NDUF3 N端抗体的跨物种反应性，证明其适用于斑马鱼模型的线粒体功能研究，为在体研究复合物I的遗传调控提供了工具。
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**注意**：以上文献为模拟内容，实际引用时需查询真实数据库(如PubMed)并核实信息。如需真实文献，建议使用关键词 **NDUF3 antibody** + **mitochondrial complex I** 进一步检索。
The NDUF3 (N-term) antibody is a monoclonal or polyclonal antibody specifically designed to target the N-terminal region of the NADH:ubiquinone oxidoreductase subunit B3 (NDUFB3), a critical component of mitochondrial Complex I (NADH dehydrogenase). Complex I is the largest enzyme in the mitochondrial electron transport chain (ETC), essential for oxidative phosphorylation and cellular ATP production. NDUFB3. encoded by the NDUFB3 gene, is a hydrophobic subunit embedded in the inner mitochondrial membrane, contributing to Complex I assembly, stability, and enzymatic activity.
This antibody is widely used in research to detect and quantify NDUFB3 expression in various biological samples, aiding studies on mitochondrial dysfunction linked to neurodegenerative disorders (e.g., Parkinson’s disease), metabolic syndromes, and cancer. By recognizing the N-terminal epitope, it helps assess protein localization via immunofluorescence or immunohistochemistry and evaluate expression changes via Western blotting or flow cytometry. Validation often includes knockdown experiments or comparisons with mitochondrial-deficient cell lines to confirm specificity.
Researchers utilize this tool to explore mechanisms underlying mitochondrial diseases, screen for therapeutic targets, or monitor Complex I integrity in metabolic studies. Its application extends to diagnostics, where altered NDUFB3 levels may serve as biomarkers for mitochondrial disorders. Proper controls, such as peptide blocking assays, are recommended to ensure antibody specificity in experimental settings.
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