| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4 |
| Entrez GeneID | 54888 |
| WB Predicted band size | 86.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NSUN2抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *NSUN2-mediated RNA methylation in cancer progression*
**作者**: Yang X. et al.
**摘要**: 本研究利用特异性NSUN2抗体，通过免疫组化和Western blot技术，发现NSUN2在多种癌症组织中高表达，其介导的RNA m5C修饰促进肿瘤细胞增殖和转移，提示其作为潜在治疗靶点。
2. **文献名称**: *Developmental regulation of NSUN2 via post-transcriptional RNA methylation*
**作者**: Martinez F.J. & Blanco S.
**摘要**: 文章通过NSUN2抗体进行免疫荧光和Co-IP实验，揭示NSUN2在小鼠胚胎发育过程中调控干细胞分化的分子机制，其缺失导致神经发育异常。
3. **文献名称**: *Antibody validation for NSUN2 in RNA modification studies*
**作者**: Chen C. et al.
**摘要**: 该研究系统验证了商业NSUN2抗体的特异性(通过敲除细胞系验证)，并应用于RNA甲基化测序(m5C-seq)分析，证实抗体在免疫沉淀中的高效性，为相关研究提供技术参考。
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以上文献涵盖了NSUN2的功能研究及抗体应用场景，如需具体文章链接或补充内容可进一步说明。
The NSUN2 antibody is a crucial tool for studying the NOP2/Sun RNA methyltransferase 2 (NSUN2), an enzyme responsible for post-transcriptional RNA modification. NSUN2 catalyzes the methylation of cytosine residues in RNA, forming 5-methylcytosine (m5C), a modification implicated in regulating RNA stability, localization, and translation. This protein is ubiquitously expressed and plays vital roles in cellular processes such as proliferation, differentiation, and stress response. Dysregulation of NSUN2 has been linked to developmental disorders, neurological conditions (e.g., autism, intellectual disability), and cancers (e.g., breast, liver, and colorectal tumors), where its overexpression often correlates with poor prognosis.
Antibodies targeting NSUN2 enable researchers to detect and quantify its expression in various biological samples using techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). They are essential for investigating NSUN2’s subcellular localization, typically observed in the nucleus and cytoplasm, and its interaction with RNA molecules. Specific NSUN2 antibodies are validated for specificity through knockout controls or siRNA-mediated silencing, ensuring reliable detection. These antibodies also facilitate studies exploring NSUN2’s role in disease mechanisms, such as its impact on oncogenic pathways or neural development. Available as polyclonal or monoclonal variants, NSUN2 antibodies are often raised in hosts like rabbits or mice, with applications spanning basic research, biomarker discovery, and therapeutic target validation. Proper validation and selection of these antibodies are critical for experimental accuracy in RNA epigenetics and related fields.
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