| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B |
| Entrez GeneID | 163486 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为3条关于DENND1B (N-term)抗体的示例参考文献(内容为虚构示例，仅用于说明格式)：
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1. **文献名称**: "DENND1B regulates T-cell receptor signaling through interaction with CLIP2"
**作者**: Smith A, et al.
**摘要**: 本研究利用DENND1B (N-term)特异性抗体，通过免疫共沉淀和Western blot技术，揭示了DENND1B在T细胞受体(TCR)信号通路中与CLIP2蛋白的相互作用，并证明其通过调控囊泡运输影响T细胞活化。
2. **文献名称**: "Expression profiling of DENND1B in autoimmune diseases using a novel N-terminal antibody"
**作者**: Chen L, et al.
**摘要**: 开发了一种针对DENND1B N端的单克隆抗体，通过免疫组化分析发现DENND1B在类风湿性关节炎患者的滑膜组织中高表达，提示其可能参与自身免疫反应的病理机制。
3. **文献名称**: "DENND1B modulates endosomal trafficking in neuronal cells"
**作者**: Tanaka K, et al.
**摘要**: 使用DENND1B (N-term)抗体进行免疫荧光定位，发现DENND1B通过调控早期内体运输影响神经元突触囊泡循环，为神经退行性疾病研究提供了新靶点。
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注：以上文献为示例性内容，实际引用需查询真实数据库(如PubMed)。如需具体文献，建议在学术平台检索关键词“DENND1B antibody N-terminal”或相关功能研究。
The DENND1B (N-term) antibody is designed to target the N-terminal region of the DENND1B protein, a member of the DENN (differentially expressed in normal and neoplastic cells) domain-containing family. DENND1B functions as a guanine nucleotide exchange factor (GEF) for Rab GTPases, particularly Rab35. playing a critical role in regulating intracellular vesicle trafficking, membrane dynamics, and receptor recycling. Its involvement in endocytic pathways and cellular signaling makes it a key player in processes like cytokinesis, immune response, and neuronal development.
Research using the DENND1B (N-term) antibody has helped elucidate the protein’s expression patterns, localization, and interactions. Studies suggest DENND1B is ubiquitously expressed, with higher levels in immune-related tissues and the brain. Aberrant DENND1B activity has been linked to diseases such as asthma, autoimmune disorders, and certain cancers, highlighting its potential as a therapeutic target. The antibody is commonly employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect DENND1B in experimental models, aiding in mechanistic studies of its role in health and disease.
The N-terminal-specific antibody is particularly valuable for distinguishing DENND1B from other DENN family proteins, ensuring specificity in experiments. Its development has advanced understanding of how DENND1B dysfunction contributes to pathological conditions, offering insights into pathways that could be modulated for treatment strategies.
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