关键信息
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基因名
KRT85
- 应用
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别名
KRT85；KRTHB5；Keratin. type II cuticular Hb5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P78386
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表达区间
1-507aa
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氨基酸序列
MSCRSYRISSGCGVTRNFSSCSAVAPKTGNRCCISAAPYRGVSCYRGLTGFGSRSLCNLGSCGPRIAVGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKWQFYQNQRCCESNLEPLFSGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVVLKKDVDCAYLRKSDLEANVEALVEESSFLRRLYEEEIRVLQAHISDTSVIVKMDNSRDLNMDCIIAEIKAQYDDVASRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEIENAKCQRAKLEAAVAEAEQQGEAALSDARCKLAELEGALQKAKQDMACLLKEYQEVMNSKLGLDIEIATYRRLLEGEEHRLCEGVGSVNVCVSSSRGGVSCGGLSYSTTPGRQITSGPSAIGGSITVVAPDSCAPCQPRSSSFSCGSSRSVRFA
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分子量
57.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KRT85, or keratin 85, is a member of the keratin family, which plays a crucial role in the structural integrity and function of epithelial tissues. Keratins are fibrous proteins that form intermediate filaments, contributing to the mechanical resilience of cells. The study of KRT85 is particularly relevant in the context of skin health and various dermatological disorders. Research has indicated that mutations or aberrant expression of keratin proteins can lead to skin diseases, including epidermolysis bullosa and other keratinopathic disorders. Moreover, KRT85 has been implicated in cellular processes such as differentiation, proliferation, and response to stress, making it a potential biomarker for skin conditions. Understanding the function and regulation of KRT85 can provide insights into the molecular mechanisms underlying skin development and pathologies. As a result, recombinant KRT85 proteins have garnered attention for use in both basic research and potential therapeutic applications. By producing KRT85 through recombinant DNA technology, researchers can explore its biological properties more comprehensively, study its interactions with other cellular components, and investigate its role in skin regeneration and repair. These studies pave the way for innovative strategies in treating skin injuries and diseases, highlighting the importance of KRT85 in dermatological research. Overall, the exploration of KRT85 as a recombinant protein not only advances our understanding of keratin biology but also holds promise for future therapeutic interventions aimed at enhancing skin health.
