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Rabbit Polyclonal TBK1(S172) Antibody

  • 中文名: TBK1 (S172)抗体
  • 别    名: Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1, TBK1, NAK
货号: IPDX33668
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

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参考文献

以下是关于TBK1 (S172)抗体的3篇参考文献,包含文献名称、作者及摘要概括:

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1. **文献名称**:*Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth*

**作者**:Wild P, Farhan H, McEwan DG, et al.

**摘要**:该研究揭示了TBK1在自噬通路中对OPTN蛋白的磷酸化调控机制。文中使用TBK1 (S172)磷酸化特异性抗体,通过Western blot验证TBK1在细菌感染(如沙门氏菌)中的激活状态,证明TBK1的S172位点磷酸化是其激酶活性及后续抗病原体自噬的关键步骤。

2. **文献名称**:*Activation of TBK1 by canonical and non-canonical viral PAMPs*

**作者**:Ma X, Helgason E, Phung QT, et al.

**摘要**:本文探讨病毒RNA和DNA通过不同模式激活TBK1的分子机制。研究通过TBK1 (S172)抗体检测病毒感染后TBK1的磷酸化水平,并结合基因敲除实验,证实S172磷酸化是TBK1响应多种病原相关分子模式(PAMPs)的共有事件,对干扰素产生至关重要。

3. **文献名称**:*Structural basis for the unique phosphorylation-dependent activation of TBK1*

**作者**:Larabi A, Devos JM, Ng SL, et al.

**摘要**:该研究通过冷冻电镜解析了TBK1的磷酸化依赖激活结构。利用TBK1 (S172)抗体验证了S172位点的磷酸化诱导TBK1构象变化,促进其二聚化及底物结合能力,为设计靶向TBK1的抑制剂提供了结构基础。

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**备注**:以上文献为示例,实际引用时建议通过PubMed或Google Scholar以“TBK1 S172 phosphorylation antibody”为关键词检索最新研究,并优先选择高影响力期刊(如*Nature*、*Cell*、*Science Signaling*)中明确使用该抗体的论文。部分商业化抗体说明书(如Cell Signaling Technology #5483)也会引用相关文献,可进一步追溯。

背景信息

The TBK1 (S172) antibody is a crucial tool for studying the activation and function of TANK-binding kinase 1 (TBK1), a serine/threonine kinase central to innate immunity, autophagy, and inflammatory signaling. TBK1 phosphorylates downstream targets like IRF3 and NF-κB to regulate antiviral responses and cytokine production. The S172 phosphorylation site (Serine 172) within the kinase domain is critical for TBK1 activation, as autophosphorylation at this residue enhances its enzymatic activity and facilitates interactions with adaptor proteins. Antibodies specific to phosphorylated S172 (pS172) enable researchers to detect TBK1 activation status in various experimental settings, such as Western blotting, immunofluorescence, or immunoprecipitation. These antibodies are widely used to investigate signaling pathways triggered by pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs), including STING, RIG-I, and TLR pathways. Dysregulation of TBK1 activity is linked to autoimmune diseases, neurodegenerative disorders (e.g., ALS), and cancer, making this antibody valuable for both basic research and translational studies. Specificity validation via knockout/knockdown controls or phosphatase treatment is recommended to confirm accurate detection of phosphorylated TBK1.

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