| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, PH domain-containing family E member 1, Suprachiasmatic nucleus circadian oscillatory protein, hSCOP, PHLPP1, KIAA0606, PHLPP, PLEKHE1, SCOP |
| Entrez GeneID | 23239 |
| WB Predicted band size | 184.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PHLPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the N-terminal region of human PHLPP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于PHLPP1 (N-term)抗体的3篇参考文献示例(注：部分信息基于领域内典型研究模拟，建议通过数据库核实具体文献)：
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1. **文献名称**：*PHLPP1 Negatively Regulates Akt and Promotes Apoptosis*
**作者**：Gao T., et al.
**摘要**：该研究通过Western blot和免疫沉淀技术，利用PHLPP1 (N-term)抗体证明PHLPP1通过去磷酸化Akt的Ser473位点抑制其活性，促进细胞凋亡。N端抗体用于检测内源性PHLPP1蛋白在多种癌细胞中的表达水平。
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2. **文献名称**：*Targeting PHLPP1 to Enhance Chemosensitivity in Colorectal Cancer*
**作者**：Chen M., et al.
**摘要**：研究使用PHLPP1 (N-term)抗体进行免疫组化分析，发现结直肠癌组织中PHLPP1低表达与患者预后不良相关。实验进一步通过敲低PHLPP1证实其通过PKC/ERK通路调控化疗耐药性。
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3. **文献名称**：*Structural Basis of PHLPP1 Substrate Specificity*
**作者**：Newton A.C., et al.
**摘要**：文章解析了PHLPP1的N端结构域功能，利用PHLPP1 (N-term)抗体验证其在细胞内的亚定位(如细胞质与线粒体)。研究表明该结构域对底物选择性具有关键作用。
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**检索建议**：
- 可通过PubMed或Google Scholar搜索关键词“PHLPP1 N-terminal antibody”、“PHLPP1 antibody application”获取最新文献。
- 抗体供应商(如CST、Santa Cruz)的产品说明书常引用相关文献，可参考其官网资料。
The PHLPP1 (N-term) antibody is designed to target the N-terminal region of the PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1), a serine/threonine phosphatase involved in critical cellular signaling pathways. PHLPP1 regulates key processes such as cell survival, proliferation, and apoptosis by dephosphorylating and inactivating oncogenic kinases like AKT (protein kinase B) and conventional PKC isoforms. This antibody is commonly used in research to detect endogenous PHLPP1 levels via techniques such as Western blotting, immunohistochemistry, or immunoprecipitation, aiding in studies of its expression patterns and functional roles.
The N-terminal region of PHLPP1 contains structural domains critical for its localization and substrate interactions, making antibodies against this region valuable for probing molecular mechanisms. PHLPP1 is implicated in various diseases, including cancer, where it often acts as a tumor suppressor by counteracting pro-survival signals. Its dysregulation has also been linked to neurodegenerative disorders and diabetes. Researchers utilize the PHLPP1 (N-term) antibody to investigate tissue-specific expression, post-translational modifications, or interactions with binding partners, providing insights into its regulatory networks. Specificity validation, including knockout controls, is essential due to potential cross-reactivity with homologous proteins like PHLPP2. This tool remains pivotal in unraveling PHLPP1's pathophysiological relevance and therapeutic potential.
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