| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tribbles homolog 1, TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1, TRIB1 (HGNC:16891) |
| Entrez GeneID | 10221 |
| WB Predicted band size | 41.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This TRIB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇关于TRIB1抗体的参考文献及其摘要概括(虚构示例，仅供参考)：
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1. **文献名称**: *"Tribbles Homolog 1 (TRIB1) Antibody Characterization in Leukemia Cell Lines"*
**作者**: Yokoyama T. et al.
**摘要**: 本研究开发了一种高特异性兔源多克隆TRIB1抗体，通过Western blot和免疫荧光验证其在AML细胞系中的表达。结果表明，TRIB1与C/EBPα蛋白相互作用，影响白血病细胞分化，抗体可用于临床样本中TRIB1异常表达的检测。
2. **文献名称**: *"A Monoclonal Antibody Targeting TRIB1 Reveals Its Role in Lipid Metabolism Regulation"*
**作者**: Smith J.R. et al.
**摘要**: 作者报道了一种小鼠单克隆TRIB1抗体(克隆号4D2)，验证其在肝脏组织中的特异性，并证明TRIB1通过调控ApoB降解影响血脂水平。该抗体适用于免疫组化及ELISA，为代谢疾病研究提供工具。
3. **文献名称**: *"TRIB1-Specific Antibody Applications in Cancer Immunohistochemistry"*
**作者**: Lee S. et al.
**摘要**: 本研究评估了商用TRIB1抗体(Abcam货号xxxxx)在结直肠癌组织中的适用性，发现其与患者预后负相关。抗体在FFPE样本中表现稳定，为TRIB1作为癌症生物标志物提供支持。
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注：以上文献为模拟内容，实际引用时请通过PubMed或Google Scholar检索真实文献(关键词：TRIB1 antibody, anti-TRIB1 validation)。
The TRIB1 (Tribbles pseudokinase 1) antibody is a research tool designed to detect and study the TRIB1 protein, a member of the Tribbles family of pseudokinases. TRIB1 functions as a scaffold protein regulating key signaling pathways, including the MAPK/ERK, PI3K/AKT, and NF-κB cascades. It interacts with proteins like MEK1. C/EBP transcription factors, and COP1 ubiquitin ligase, influencing cell proliferation, differentiation, and apoptosis. Dysregulation of TRIB1 is implicated in cancers, metabolic disorders (e.g., dyslipidemia), and inflammatory diseases.
TRIB1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, localization, and interactions. These antibodies help elucidate TRIB1's role in disease mechanisms, such as its oncogenic potential in leukemia or its regulatory effects on lipid metabolism. Researchers also utilize TRIB1 antibodies to validate CRISPR/KO models or assess post-translational modifications. Commercial TRIB1 antibodies are typically raised against specific epitopes (e.g., human TRIB1 amino acids 150-250), with validation in knockout controls to ensure specificity. Challenges include cross-reactivity with homologous family members (TRIB2. TRIB3), emphasizing the need for rigorous validation in experimental systems.
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