| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT |
| Entrez GeneID | 11130 |
| WB Predicted band size | 31.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ZWINT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the Central region of human ZWINT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
+ +
以下是3篇涉及ZWINT抗体的参考文献及其摘要内容概括：
1. **文献名称**: "ZWINT: A key regulator of kinetochore assembly and mitotic progression"
**作者**: Wang et al.
**摘要**: 研究揭示了ZWINT蛋白通过与ZW10和KNL1复合物相互作用，在调控动粒组装和染色体分离中的关键作用。文中使用特异性ZWINT抗体进行免疫沉淀和免疫荧光实验，证实其在细胞周期依赖性定位及功能。
2. **文献名称**: "Overexpression of ZWINT is associated with poor prognosis in hepatocellular carcinoma"
**作者**: Li et al.
**摘要**: 通过免疫组化(使用商业化ZWINT抗体)分析肝细胞癌组织样本，发现ZWINT高表达与肿瘤进展、转移及患者生存率降低显著相关，提示其作为潜在预后标志物的价值。
3. **文献名称**: "Interaction between ZWINT and the MIS12 complex regulates mitotic checkpoint signaling"
**作者**: Zhang & Liu
**摘要**: 研究利用ZWINT抗体进行共免疫沉淀和蛋白质质谱分析，发现ZWINT与MIS12复合物相互作用，调控纺锤体组装检查点(SAC)信号传导，影响细胞周期停滞和基因组稳定性。
4. **文献名称**: "ZWINT as a novel therapeutic target in triple-negative breast cancer"
**作者**: Chen et al.
**摘要**: 通过Western blot和免疫荧光(使用兔源ZWINT抗体)发现，ZWINT在三阴性乳腺癌中异常高表达，其敲低显著抑制肿瘤细胞增殖并增强化疗敏感性，提示靶向ZWINT的治疗潜力。
---
以上文献均为示例，实际发表信息需通过PubMed或专业数据库核实。ZWINT抗体相关研究多集中于其在有丝分裂、癌症发生及诊断中的应用。
**Background of ZWINT Antibody**
ZWINT (ZW10 Interacting Kinase) is a protein that plays a critical role in the mitotic checkpoint, a surveillance mechanism ensuring accurate chromosome segregation during cell division. It interacts with ZW10. a component of the RZZ (ROD-ZWILCH-ZW10) complex, which is essential for recruiting dynein-dynactin motors to kinetochores. This interaction facilitates the removal of checkpoint proteins from kinetochores once chromosomes achieve proper microtubule attachment, enabling mitotic progression.
ZWINT antibodies are valuable tools for studying the molecular mechanisms of chromosome alignment, spindle assembly checkpoint (SAC) signaling, and genomic stability. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect ZWINT expression, localization, and protein-protein interactions. Dysregulation of ZWINT has been implicated in cancers, including breast and hepatocellular carcinoma, where its overexpression correlates with tumor progression and poor prognosis.
Studies involving ZWINT antibodies have also shed light on its role in maintaining kinetochore integrity and regulating Aurora B kinase activity. Specificity and validation of these antibodies (e.g., via knockout controls) are crucial for reliable experimental outcomes. Overall, ZWINT antibodies serve as essential reagents for exploring cell cycle regulation, mitotic errors, and potential therapeutic targets in cancer biology.
×
